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1gz6

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[[Image:1gz6.gif|left|200px]]<br />
 
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<applet load="1gz6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gz6, resolution 2.38&Aring;" />
 
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'''(3R)-HYDROXYACYL-COA DEHYDROGENASE FRAGMENT OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 2'''<br />
 
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==Overview==
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==(3R)-HYDROXYACYL-COA DEHYDROGENASE FRAGMENT OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 2==
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The crystal structure of (3R)-hydroxyacyl-CoA dehydrogenase of rat, peroxisomal multifunctional enzyme type 2 (MFE-2) was solved at 2.38 A, resolution. The catalytic entity reveals an alpha/beta short chain alcohol, dehydrogenase/reductase (SDR) fold and the conformation of the bound, nicotinamide adenine dinucleotide (NAD(+)) found in other SDR enzymes. Of, great interest is the separate COOH-terminal domain, which is not seen in, other SDR structures. This domain completes the active site cavity of the, neighboring monomer and extends dimeric interactions. Peroxisomal diseases, that arise because of point mutations in the dehydrogenase-coding region, of the MFE-2 gene can be mapped to changes in amino acids involved in, NAD(+) binding and protein dimerization.
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<StructureSection load='1gz6' size='340' side='right'caption='[[1gz6]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gz6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GZ6 FirstGlance]. <br>
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1GZ6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]] with SO4 and NAI as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Estradiol_17-beta-dehydrogenase Estradiol 17-beta-dehydrogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.62 1.1.1.62]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZ6 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gz6 OCA], [https://pdbe.org/1gz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gz6 RCSB], [https://www.ebi.ac.uk/pdbsum/1gz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gz6 ProSAT]</span></td></tr>
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Binary structure of the two-domain (3R)-hydroxyacyl-CoA dehydrogenase from rat peroxisomal multifunctional enzyme type 2 at 2.38 A resolution., Haapalainen AM, Koski MK, Qin YM, Hiltunen JK, Glumoff T, Structure. 2003 Jan;11(1):87-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12517343 12517343]
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</table>
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[[Category: Estradiol 17-beta-dehydrogenase]]
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== Function ==
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[https://www.uniprot.org/uniprot/DHB4_RAT DHB4_RAT] Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/1gz6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gz6 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Glumoff T]]
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[[Category: Glumoff, T.]]
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[[Category: Haapalainen AM]]
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[[Category: Haapalainen, A.M.]]
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[[Category: Hiltunen JK]]
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[[Category: Hiltunen, J.K.]]
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[[Category: NAI]]
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[[Category: SO4]]
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[[Category: 17beta-hsd4]]
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[[Category: beta-oxidation]]
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[[Category: mfe-2]]
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[[Category: multigene famil]]
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[[Category: nadp]]
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[[Category: oxidoreductase]]
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[[Category: peroxisome]]
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[[Category: sdr]]
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[[Category: steroid biosynthesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:56:17 2007''
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Current revision

(3R)-HYDROXYACYL-COA DEHYDROGENASE FRAGMENT OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 2

PDB ID 1gz6

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