1h32

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[[Image:1h32.jpg|left|200px]]<br /><applet load="1h32" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h32, resolution 1.5&Aring;" />
 
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'''REDUCED SOXAX COMPLEX FROM RHODOVULUM SULFIDOPHILUM'''<br />
 
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==Overview==
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==Reduced SoxAX complex from Rhodovulum sulfidophilum==
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Reduced inorganic sulfur compounds are utilized by many bacteria as, electron donors to photosynthetic or respiratory electron transport, chains. This metabolism is a key component of the biogeochemical sulfur, cycle. The SoxAX protein is a heterodimeric c-type cytochrome involved in, thiosulfate oxidation. The crystal structures of SoxAX from the, photosynthetic bacterium Rhodovulum sulfidophilum have been solved at 1.75, A resolution in the oxidized state and at 1.5 A resolution in the, dithionite-reduced state, providing the first structural insights into the, enzymatic oxidation of thiosulfate. The SoxAX active site contains a haem, with unprecedented cysteine persulfide (cysteine sulfane) coordination., This unusual post-translational modification is also seen in, sulfurtransferases such as rhodanese. Intriguingly, this enzyme shares, further active site characteristics with SoxAX such as an adjacent, conserved arginine residue and a strongly positive electrostatic, potential. These similarities have allowed us to suggest a catalytic, mechanism for enzymatic thiosulfate oxidation. The atomic coordinates and, experimental structure factors have been deposited in the PDB with the, accession codes 1H31, 1H32 and 1H33.
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<StructureSection load='1h32' size='340' side='right'caption='[[1h32]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1h32]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodovulum_sulfidophilum Rhodovulum sulfidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H32 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h32 OCA], [https://pdbe.org/1h32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h32 RCSB], [https://www.ebi.ac.uk/pdbsum/1h32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h32 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SOXA_RHOSU SOXA_RHOSU] C-type diheme cytochrome, which is part of the SoxAX cytochrome complex involved in sulfur oxidation. The SoxAX complex catalyzes the formation of a heterodisulfide bond between the conserved cysteine residue on a sulfur carrier SoxYZ complex subunit SoxY and thiosulfate or other inorganic sulfur substrates. This leads to the liberation of two electrons, which may be transferred from the SoxAX complex to another cytochrome c and which then may be used for reductive CO(2) fixation.<ref>PMID:11523998</ref> <ref>PMID:11567011</ref> <ref>PMID:12411478</ref> <ref>PMID:23060437</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h3/1h32_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h32 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1H32 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodovulum_sulfidophilum Rhodovulum sulfidophilum] with <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=HEC:'>HEC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=EA1:Hec+Binding+Site+For+Chain+B'>EA1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H32 OCA].
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*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural basis for the oxidation of thiosulfate by a sulfur cycle enzyme., Bamford VA, Bruno S, Rasmussen T, Appia-Ayme C, Cheesman MR, Berks BC, Hemmings AM, EMBO J. 2002 Nov 1;21(21):5599-610. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12411478 12411478]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rhodovulum sulfidophilum]]
[[Category: Rhodovulum sulfidophilum]]
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[[Category: Appia-Ayme, C.]]
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[[Category: Appia-Ayme C]]
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[[Category: Bamford, V.A.]]
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[[Category: Bamford VA]]
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[[Category: Berks, B.C.]]
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[[Category: Berks BC]]
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[[Category: Bruno, S.]]
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[[Category: Bruno S]]
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[[Category: Cheesman, M.R.]]
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[[Category: Cheesman MR]]
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[[Category: Hemmings, A.M.]]
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[[Category: Hemmings AM]]
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[[Category: Rasmussen, T.]]
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[[Category: Rasmussen T]]
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[[Category: EDO]]
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[[Category: HEC]]
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[[Category: cysteine persulfide heme ligand]]
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[[Category: cytochrome c]]
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[[Category: soxax complex]]
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[[Category: sulfur cycle]]
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[[Category: thiosulfate oxidation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:45:41 2008''
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Current revision

Reduced SoxAX complex from Rhodovulum sulfidophilum

PDB ID 1h32

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