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1h34

From Proteopedia

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[[Image:1h34.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of lima bean trypsin inhibitor==
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|PDB= 1h34 |SIZE=350|CAPTION= <scene name='initialview01'>1h34</scene>, resolution 2.04&Aring;
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<StructureSection load='1h34' size='340' side='right'caption='[[1h34]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=GLX:GLU/GLN+AMBIGUOUS'>GLX</scene>
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<table><tr><td colspan='2'>[[1h34]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H34 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLX:GLU/GLN+AMBIGUOUS'>GLX</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h34 OCA], [https://pdbe.org/1h34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h34 RCSB], [https://www.ebi.ac.uk/pdbsum/1h34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h34 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h34 OCA], [http://www.ebi.ac.uk/pdbsum/1h34 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h34 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/IBB_PHALU IBB_PHALU]
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== Evolutionary Conservation ==
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'''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h3/1h34_consurf.spt"</scriptWhenChecked>
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SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1H34 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_lunatus Phaseolus lunatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H34 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h34 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case., Debreczeni JE, Bunkoczi G, Girmann B, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):393-5. Epub 2003, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12554963 12554963]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Phaseolus lunatus]]
[[Category: Phaseolus lunatus]]
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[[Category: Single protein]]
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[[Category: Bunkoczi G]]
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[[Category: Bunkoczi, G.]]
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[[Category: Debreczeni JE]]
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[[Category: Debreczeni, J E.]]
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[[Category: Girmann B]]
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[[Category: Girmann, B.]]
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[[Category: Sheldrick GM]]
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[[Category: Sheldrick, G M.]]
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[[Category: bowman-birk-type proteinase inhibitor]]
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[[Category: serine protease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:56:45 2008''
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Current revision

Crystal structure of lima bean trypsin inhibitor

PDB ID 1h34

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