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1h3g

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[[Image:1h3g.gif|left|200px]]<br />
 
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<applet load="1h3g" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h3g, resolution 2.1&Aring;" />
 
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'''CYCLOMALTODEXTRINASE FROM FLAVOBACTERIUM SP. NO. 92: FROM DNA SEQUENCE TO PROTEIN STRUCTURE'''<br />
 
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==Overview==
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==Cyclomaltodextrinase from Flavobacterium sp. No. 92: from DNA sequence to protein structure==
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Starting with oligopeptide sequences and using PCR, the gene of the, cyclodextrinase from Flavobacterium sp. no. 92 was derived from the, genomic DNA. The gene was sequenced and expressed in Escherichia coli; the, gene product was purified and crystallized. An X-ray diffraction analysis, using seleno-methionines with multiwavelength anomalous diffraction, techniques yielded the refined 3D structure at 2.1 A resolution. The, enzyme hydrolyzes alpha(1,4)-glycosidic bonds of cyclodextrins and linear, malto-oligosaccharides. It belongs to the glycosylhydrolase family no. 13, and has a chain fold similar to that of alpha-amylases, cyclodextrin, glycosyltransferases, and other cyclodextrinases. In contrast with most, family members but in agreement with other cyclodextrinases, the enzyme, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12752453 (full description)]]
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<StructureSection load='1h3g' size='340' side='right'caption='[[1h3g]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h3g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavobacterium_sp._92 Flavobacterium sp. 92]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H3G FirstGlance]. <br>
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1H3G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Flavobacterium_sp._92 Flavobacterium sp. 92]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Cyclomaltodextrinase Cyclomaltodextrinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.54 3.2.1.54]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H3G OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h3g OCA], [https://pdbe.org/1h3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h3g RCSB], [https://www.ebi.ac.uk/pdbsum/1h3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h3g ProSAT]</span></td></tr>
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Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92., Fritzsche HB, Schwede T, Schulz GE, Eur J Biochem. 2003 May;270(10):2332-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12752453 12752453]
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</table>
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[[Category: Cyclomaltodextrinase]]
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== Function ==
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[https://www.uniprot.org/uniprot/Q8KKG0_9FLAO Q8KKG0_9FLAO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h3/1h3g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h3g ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Flavobacterium sp. 92]]
[[Category: Flavobacterium sp. 92]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fritzsche, H.B.]]
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[[Category: Fritzsche HB]]
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[[Category: Jelakovic, S.]]
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[[Category: Jelakovic S]]
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[[Category: Schulz, G.E.]]
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[[Category: Schulz GE]]
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[[Category: Schwede, T.]]
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[[Category: Schwede T]]
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[[Category: CA]]
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[[Category: cyclomaltodextrinase]]
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[[Category: glycosidase]]
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[[Category: hydrolase]]
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[[Category: signal]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:27:29 2007''
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Current revision

Cyclomaltodextrinase from Flavobacterium sp. No. 92: from DNA sequence to protein structure

PDB ID 1h3g

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