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1h4i

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[[Image:1h4i.gif|left|200px]]
 
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{{Structure
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==Methylobacterium extorquens methanol dehydrogenase==
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|PDB= 1h4i |SIZE=350|CAPTION= <scene name='initialview01'>1h4i</scene>, resolution 1.94&Aring;
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<StructureSection load='1h4i' size='340' side='right'caption='[[1h4i]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Pqq+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Ca+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Pqq+Binding+Site+For+Chain+C'>AC3</scene> and <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+C'>AC4</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=PQQ:PYRROLOQUINOLINE QUINONE'>PQQ</scene>
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<table><tr><td colspan='2'>[[1h4i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4I FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4i OCA], [https://pdbe.org/1h4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4i RCSB], [https://www.ebi.ac.uk/pdbsum/1h4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHM1_METEA DHM1_METEA] Catalyzes the oxidation of primary alcohols including methanol.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h4i ConSurf].
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<div style="clear:both"></div>
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'''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE'''
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==See Also==
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*[[Methanol dehydrogenase|Methanol dehydrogenase]]
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__TOC__
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==Overview==
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</StructureSection>
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BACKGROUND: Methanol dehydrogenase (MDH) is a bacterial periplasmic quinoprotein; it has pyrrolo-quinoline quinone (PQQ) as its prosthetic group, requires Ca2+ for activity and uses cytochrome cL as its electron acceptor. Low-resolution structures of MDH have already been determined. RESULTS: The structure of the alpha 2 beta 2 tetramer of MDH from Methylobacterium extorquens has now been determined at 1.94 A with an R-factor of 19.85%. CONCLUSIONS: The alpha-subunit of MDH has an eight-fold radial symmetry, with its eight beta-sheets stabilized by a novel tryptophan docking motif. The PQQ in the active site is held in place by a coplanar tryptophan and by a novel disulphide ring formed between adjacent cysteines which are bonded by an unusual non-planar trans peptide bond. One of the carbonyl oxygens of PQQ is bonded to the Ca2+, probably facilitating attack on the substrate, and the other carbonyl oxygen is out of the plane of the ring, confirming the presence of the predicted free-radical semiquinone form of the prosthetic group.
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[[Category: Large Structures]]
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[[Category: Methylorubrum extorquens]]
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==About this Structure==
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[[Category: Anthony C]]
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1H4I is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA].
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[[Category: Blake C]]
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[[Category: Ghosh M]]
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==Reference==
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[[Category: Goodwin MG]]
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The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7735834 7735834]
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[[Category: Harlos K]]
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[[Category: Alcohol dehydrogenase (acceptor)]]
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[[Category: Methylobacterium extorquens]]
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[[Category: Protein complex]]
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[[Category: Anthony, C.]]
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[[Category: Blake, C.]]
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[[Category: Ghosh, M.]]
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[[Category: Goodwin, M G.]]
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[[Category: Harlos, K.]]
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[[Category: CA]]
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[[Category: PQQ]]
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[[Category: dehydrogenase]]
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[[Category: quinoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:32:04 2008''
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Current revision

Methylobacterium extorquens methanol dehydrogenase

PDB ID 1h4i

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