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1h4i

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(New page: 200px<br /> <applet load="1h4i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h4i, resolution 1.94&Aring;" /> '''METHYLOBACTERIUM EX...)
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[[Image:1h4i.gif|left|200px]]<br />
 
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<applet load="1h4i" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h4i, resolution 1.94&Aring;" />
 
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'''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE'''<br />
 
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==Overview==
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==Methylobacterium extorquens methanol dehydrogenase==
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BACKGROUND: Methanol dehydrogenase (MDH) is a bacterial periplasmic, quinoprotein; it has pyrrolo-quinoline quinone (PQQ) as its prosthetic, group, requires Ca2+ for activity and uses cytochrome cL as its electron, acceptor. Low-resolution structures of MDH have already been determined., RESULTS: The structure of the alpha 2 beta 2 tetramer of MDH from, Methylobacterium extorquens has now been determined at 1.94 A with an, R-factor of 19.85%. CONCLUSIONS: The alpha-subunit of MDH has an, eight-fold radial symmetry, with its eight beta-sheets stabilized by a, novel tryptophan docking motif. The PQQ in the active site is held in, place by a coplanar tryptophan and by a novel disulphide ring formed, between adjacent cysteines which are bonded by an unusual non-planar trans, peptide bond. One ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7735834 (full description)]]
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<StructureSection load='1h4i' size='340' side='right'caption='[[1h4i]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1h4i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4i OCA], [https://pdbe.org/1h4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4i RCSB], [https://www.ebi.ac.uk/pdbsum/1h4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHM1_METEA DHM1_METEA] Catalyzes the oxidation of primary alcohols including methanol.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h4i ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1H4I is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]] with CA and PQQ as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA]].
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*[[Methanol dehydrogenase|Methanol dehydrogenase]]
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__TOC__
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==Reference==
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</StructureSection>
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The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7735834 7735834]
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[[Category: Large Structures]]
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[[Category: Methylobacterium extorquens]]
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[[Category: Methylorubrum extorquens]]
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[[Category: Protein complex]]
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[[Category: Anthony C]]
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[[Category: Anthony, C.]]
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[[Category: Blake C]]
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[[Category: Blake, C.]]
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[[Category: Ghosh M]]
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[[Category: Ghosh, M.]]
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[[Category: Goodwin MG]]
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[[Category: Goodwin, M.G.]]
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[[Category: Harlos K]]
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[[Category: Harlos, K.]]
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[[Category: CA]]
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[[Category: PQQ]]
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[[Category: dehydrogenase]]
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[[Category: quinoprotein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:38:46 2007''
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Current revision

Methylobacterium extorquens methanol dehydrogenase

PDB ID 1h4i

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