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1h4u

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(New page: 200px<br /><applet load="1h4u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h4u, resolution 2.20&Aring;" /> '''DOMAIN G2 OF MOUSE N...)
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[[Image:1h4u.gif|left|200px]]<br /><applet load="1h4u" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h4u, resolution 2.20&Aring;" />
 
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'''DOMAIN G2 OF MOUSE NIDOGEN-1'''<br />
 
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==Overview==
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==Domain G2 of mouse nidogen-1==
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Nidogen, an invariant component of basement membranes, is a, multifunctional protein that interacts with most other major basement, membrane proteins. Here, we report the crystal structure of the mouse, nidogen-1 G2 fragment, which contains binding sites for collagen IV and, perlecan. The structure is composed of an EGF-like domain and an, 11-stranded beta-barrel with a central helix. The beta-barrel domain has, unexpected similarity to green fluorescent protein. A large surface patch, on the beta-barrel is strikingly conserved in all metazoan nidogens., Site-directed mutagenesis demonstrates that the conserved residues are, involved in perlecan binding.
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<StructureSection load='1h4u' size='340' side='right'caption='[[1h4u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h4u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4U FirstGlance]. <br>
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1H4U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H4U OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4u OCA], [https://pdbe.org/1h4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4u RCSB], [https://www.ebi.ac.uk/pdbsum/1h4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4u ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1., Hopf M, Gohring W, Ries A, Timpl R, Hohenester E, Nat Struct Biol. 2001 Jul;8(7):634-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11427896 11427896]
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== Function ==
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[https://www.uniprot.org/uniprot/NID1_MOUSE NID1_MOUSE] Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h4u ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Gohring W]]
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[[Category: Gohring, W.]]
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[[Category: Hohenester E]]
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[[Category: Hohenester, E.]]
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[[Category: Hopf M]]
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[[Category: Hopf, M.]]
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[[Category: Ries A]]
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[[Category: Ries, A.]]
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[[Category: Timpl R]]
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[[Category: Timpl, R.]]
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[[Category: extracellular matrix protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:25:20 2007''
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Current revision

Domain G2 of mouse nidogen-1

PDB ID 1h4u

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