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1h4x

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[[Image:1h4x.gif|left|200px]]<br />
 
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<applet load="1h4x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h4x, resolution 1.16&Aring;" />
 
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'''STRUCTURE OF THE BACILLUS CELL FATE DETERMINANT SPOIIAA IN THE PHOSPHORYLATED FORM'''<br />
 
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==Overview==
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==Structure of the Bacillus Cell Fate Determinant SpoIIAA in the Phosphorylated Form==
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BACKGROUND: The asymmetric cell division during sporulation in Bacillus, subtilis gives rise to two compartments: the mother cell and the, forespore. Each follow different programs of gene expression coordinated, by a succession of alternate RNA polymerase sigma factors. The activity of, the first of these sigma factors, sigmaF, is restricted to the forespore, although sigmaF is present in the predivisional cell and partitions into, both compartments following the asymmetric septation. For sigmaF to become, active, it must escape from a complex with its cognate anti-sigma factor, SpoIIAB. This relief from SpoIIAB inhibition requires the, dephosphorylation of the anti-sigma factor antagonist, SpoIIAA. The, phosphorylation state of SpoIIAA is thus a key determinant of sigmaF, activity and cell fate. RESULTS: We have solved the crystal structures of, SpoIIAA from Bacillus sphaericus in its phosphorylated and, unphosphorylated forms. The overall structure consists of a central, beta-pleated sheet, one face of which is buried by a pair of alpha, helices, while the other is largely exposed to solvent. The site of, phosphorylation, Ser57, is located at the N terminus of helix alpha2. The, phosphoserine is exceptionally well defined in the 1.2 A electron density, maps, revealing that the structural changes accompanying phosphorylation, are slight. CONCLUSIONS: Comparison of unphosphorylated and phosphorylated, SpoIIAA shows that covalent modification has no significant effect on the, global structure of the protein. The phosphoryl group has a passive role, as a negatively charged flag rather than the active role it plays as a, nucleus of structural reorganization in many eukaryotic signaling systems.
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<StructureSection load='1h4x' size='340' side='right'caption='[[1h4x]], [[Resolution|resolution]] 1.16&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1h4x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.16&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4x OCA], [https://pdbe.org/1h4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4x RCSB], [https://www.ebi.ac.uk/pdbsum/1h4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SP2AA_LYSSH SP2AA_LYSSH] In the phosphorylated form it could act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma f from inhibition.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h4x ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1H4X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus] with TRS as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H4X OCA].
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*[[Anti-sigma factor antagonist 3D structures|Anti-sigma factor antagonist 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the Bacillus cell fate determinant SpoIIAA in phosphorylated and unphosphorylated forms., Seavers PR, Lewis RJ, Brannigan JA, Verschueren KH, Murshudov GN, Wilkinson AJ, Structure. 2001 Jul 3;9(7):605-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11470435 11470435]
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[[Category: Large Structures]]
[[Category: Lysinibacillus sphaericus]]
[[Category: Lysinibacillus sphaericus]]
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[[Category: Single protein]]
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[[Category: Brannigan JA]]
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[[Category: Brannigan, J.A.]]
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[[Category: Lewis RJ]]
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[[Category: Lewis, R.J.]]
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[[Category: Murshudov GN]]
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[[Category: Murshudov, G.N.]]
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[[Category: Seavers PR]]
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[[Category: Seavers, P.R.]]
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[[Category: Verschueren KHG]]
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[[Category: Verschueren, K.H.G.]]
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[[Category: Wilkinson AJ]]
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[[Category: Wilkinson, A.J.]]
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[[Category: TRS]]
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[[Category: cell differentiation]]
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[[Category: crystallography]]
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[[Category: phosphorylation]]
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[[Category: sigma factor]]
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[[Category: sporulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:10:47 2007''
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Current revision

Structure of the Bacillus Cell Fate Determinant SpoIIAA in the Phosphorylated Form

PDB ID 1h4x

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