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1h5m

From Proteopedia

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[[Image:1h5m.jpg|left|200px]]
 
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{{Structure
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==X-ray induced reduction of horseradish peroxidase C1A Compound III (0-100% dose)==
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|PDB= 1h5m |SIZE=350|CAPTION= <scene name='initialview01'>1h5m</scene>, resolution 1.60&Aring;
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<StructureSection load='1h5m' size='340' side='right'caption='[[1h5m]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE= <scene name='pdbsite=CAA:Hem+Binding+Site+For+Chain+A'>CAA</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEO:HYDROGEN+PEROXIDE'>PEO</scene>
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<table><tr><td colspan='2'>[[1h5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H5M FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEO:HYDROGEN+PEROXIDE'>PEO</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h5m OCA], [https://pdbe.org/1h5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h5m RCSB], [https://www.ebi.ac.uk/pdbsum/1h5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h5m ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h5m OCA], [http://www.ebi.ac.uk/pdbsum/1h5m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h5m RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PER1A_ARMRU PER1A_ARMRU] Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
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== Evolutionary Conservation ==
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'''X-RAY INDUCED REDUCTION OF HORSERADISH PEROXIDASE C1A COMPOUND III (0-100% DOSE)'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h5/1h5m_consurf.spt"</scriptWhenChecked>
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A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1H5M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5M OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h5m ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12024218 12024218]
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</StructureSection>
[[Category: Armoracia rusticana]]
[[Category: Armoracia rusticana]]
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[[Category: Peroxidase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Berglund GI]]
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[[Category: Berglund, G I.]]
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[[Category: Carlsson GH]]
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[[Category: Carlsson, G H.]]
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[[Category: Hajdu J]]
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[[Category: Hajdu, J.]]
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[[Category: Henriksen A]]
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[[Category: Henriksen, A.]]
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[[Category: Smith AT]]
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[[Category: Smith, A T.]]
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[[Category: Szoke H]]
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[[Category: Szoke, H.]]
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[[Category: compound iii]]
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[[Category: horseradish]]
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[[Category: oxidoreductase]]
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[[Category: oxyperoxidase]]
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[[Category: peroxidase]]
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[[Category: x-ray induced reduction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:58:12 2008''
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Current revision

X-ray induced reduction of horseradish peroxidase C1A Compound III (0-100% dose)

PDB ID 1h5m

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