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1h65

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[[Image:1h65.gif|left|200px]]
 
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==Crystal structure of pea Toc34 - a novel GTPase of the chloroplast protein translocon==
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The line below this paragraph, containing "STRUCTURE_1h65", creates the "Structure Box" on the page.
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<StructureSection load='1h65' size='340' side='right'caption='[[1h65]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1h65]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H65 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1h65| PDB=1h65 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h65 OCA], [https://pdbe.org/1h65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h65 RCSB], [https://www.ebi.ac.uk/pdbsum/1h65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h65 ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF PEA TOC34-A NOVEL GTPASE OF THE CHLOROPLAST PROTEIN TRANSLOCON'''
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== Function ==
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[https://www.uniprot.org/uniprot/TOC34_PEA TOC34_PEA] GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Toc34, a 34-kDa integral membrane protein, is a member of the Toc (translocon at the outer-envelope membrane of chloroplasts) complex, which associates with precursor proteins during protein transport across the chloroplast outer membrane. Here we report the 2.0 A resolution crystal structure of the cytosolic part of pea Toc34 in complex with GDP and Mg2+. In the crystal, Toc34 molecules exist as dimers with features resembling those found in a small GTPase in complex with a GTPase activating protein (GAP). However, gel filtration experiments revealed that dimeric and monomeric forms of Toc34 coexisted in phosphate saline buffer solution at pH 7.2. Mutation of Arg 128, an essential residue for dimerization, to an Ala residue led to the formation of an exclusively monomeric species whose GTPase activity is significantly reduced compared to that of wild type Toc34. These results, together with a number of structural features unique to Toc34, suggest that each monomer acts as a GAP on the other interacting monomer.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/1h65_consurf.spt"</scriptWhenChecked>
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1H65 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H65 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of pea Toc34, a novel GTPase of the chloroplast protein translocon., Sun YJ, Forouhar F, Li Hm HM, Tu SL, Yeh YH, Kao S, Shr HL, Chou CC, Chen C, Hsiao CD, Nat Struct Biol. 2002 Feb;9(2):95-100. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11753431 11753431]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h65 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pisum sativum]]
[[Category: Pisum sativum]]
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[[Category: Single protein]]
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[[Category: Chou CC]]
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[[Category: Chou, C C.]]
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[[Category: Forouhar F]]
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[[Category: Forouhar, F.]]
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[[Category: Hsiao CD]]
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[[Category: Hsiao, C D.]]
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[[Category: Kao S]]
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[[Category: Kao, S.]]
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[[Category: Li HM]]
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[[Category: Li, H M.]]
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[[Category: Shr HL]]
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[[Category: Shr, H L.]]
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[[Category: Sun YJ]]
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[[Category: Sun, Y J.]]
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[[Category: Tu SL]]
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[[Category: TU, S L.]]
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[[Category: Chloroplast]]
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[[Category: Gtpase]]
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[[Category: Translocon]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:28:57 2008''
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Current revision

Crystal structure of pea Toc34 - a novel GTPase of the chloroplast protein translocon

PDB ID 1h65

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