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1h75

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[[Image:1h75.gif|left|200px]]<br /><applet load="1h75" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h75, resolution 1.7&Aring;" />
 
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'''STRUCTURAL BASIS FOR THE THIOREDOXIN-LIKE ACTIVITY PROFILE OF THE GLUTAREDOXIN-LIKE PROTEIN NRDH-REDOXIN FROM ESCHERICHIA COLI.'''<br />
 
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==Overview==
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==Structural basis for the thioredoxin-like activity profile of the glutaredoxin-like protein NrdH-redoxin from Escherichia coli.==
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NrdH-redoxin is a representative of a class of small redox proteins that, contain a conserved CXXC motif and are characterized by a, glutaredoxin-like amino acid sequence and thioredoxin-like activity, profile. The crystal structure of recombinant Escherichia coli, NrdH-redoxin in the oxidized state has been determined at 1.7 A resolution, by multiwavelength anomalous diffraction. NrdH-redoxin belongs to the, thioredoxin superfamily and is structurally most similar to E. coli, glutaredoxin 3 and phage T4 glutaredoxin. The angle between the C-terminal, helix alpha3 and strand beta4, which differs between thioredoxin and, glutaredoxin, has an intermediate value in NrdH-redoxin. The orientation, of this helix is to a large extent determined by an extended hydrogen-bond, network involving the highly conserved sequence motif (61)WSGFRP(D/E)(67), which is unique to this subclass of the thioredoxin superfamily. Residues, that bind glutathione in glutaredoxins are in general not conserved in, NrdH-redoxin, and no glutathione-binding cleft is present. Instead, NrdH-redoxin contains a wide hydrophobic pocket at the surface, similar to, thioredoxin. Modeling studies suggest that NrdH-redoxin can interact with, E. coli thioredoxin reductase at this pocket and also via a loop that is, complementary to a crevice in the reductase in a similar manner as, observed in the E. coli thioredoxin-thioredoxin reductase complex.
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<StructureSection load='1h75' size='340' side='right'caption='[[1h75]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h75]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H75 FirstGlance]. <br>
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1H75 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Known structural/functional Site: <scene name='pdbsite=DIS:Redox-Active+Disulfide'>DIS</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H75 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h75 OCA], [https://pdbe.org/1h75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h75 RCSB], [https://www.ebi.ac.uk/pdbsum/1h75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h75 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structural basis for the thioredoxin-like activity profile of the glutaredoxin-like NrdH-redoxin from Escherichia coli., Stehr M, Schneider G, Aslund F, Holmgren A, Lindqvist Y, J Biol Chem. 2001 Sep 21;276(38):35836-41. Epub 2001 Jul 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11441020 11441020]
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== Function ==
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[https://www.uniprot.org/uniprot/NRDH_ECOLI NRDH_ECOLI] Electron transport system for the ribonucleotide reductase system NrdEF.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h7/1h75_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h75 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aslund, F.]]
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[[Category: Aslund F]]
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[[Category: Holmgren, A.]]
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[[Category: Holmgren A]]
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[[Category: Lindqvist, Y.]]
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[[Category: Lindqvist Y]]
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[[Category: Schneider, G.]]
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[[Category: Schneider G]]
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[[Category: Stehr, M.]]
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[[Category: Stehr M]]
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[[Category: glutaredoxin]]
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[[Category: nrdh]]
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[[Category: redox protein]]
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[[Category: thioredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:47:15 2008''
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Current revision

Structural basis for the thioredoxin-like activity profile of the glutaredoxin-like protein NrdH-redoxin from Escherichia coli.

PDB ID 1h75

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