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1h8s

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Three-dimensional structure of anti-ampicillin single chain Fv fragment complexed with the hapten.

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Retrieved from "http://52.214.119.220/wiki/index.php/1h8s"

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-[[Image:1h8s.gif|left|200px]]<br />
-<applet load="1h8s" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1h8s, resolution 2.4&Aring;" />
-'''THREE-DIMENSIONAL STRUCTURE OF ANTI-AMPICILLIN SINGLE CHAIN FV FRAGMENT COMPLEXED WITH THE HAPTEN.'''<br />
-==Overview==
+==Three-dimensional structure of anti-ampicillin single chain Fv fragment complexed with the hapten.==
-Single-chain Fv (scFv) antibody libraries were constructed from mice, immunized with an ampicillin-bovine serum albumin conjugate. Several, antibodies with specificity for intact ampicillin were selected by phage, display and characterized. The antibody scFv fragment aL2 binds to intact, ampicillin and shows no detectable cross-reactivity with hydrolyzed, ampicillin. We determined the X-ray structures of two crystal forms of, w.t. aL2, which differ mainly in the side-chain conformation of Trp H109, (according to a new consensus nomenclature Kabat residue number H95) in, the extremely short (three residues) CDR H3 and the presence or absence of, a well-resolved molecule of 2-methyl-pentane-2,4-diol in the bottom of the, binding pocket. Attempts to co-crystallize aL2 with its antigen or to, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11397088 (full description)]]
+<StructureSection load='1h8s' size='340' side='right'caption='[[1h8s]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1h8s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H8S FirstGlance]. <br>
-H8S is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mouse_e_010090 Mouse e 010090]] with SO4 and AIC as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8S OCA]].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AIC:(2S,5R,6R)-6-{[(2R)-2-AMINO-2-PHENYLETHANOYL]AMINO}-3,3-DIMETHYL-7-OXO-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC+ACID'>AIC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h8s OCA], [https://pdbe.org/1h8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h8s RCSB], [https://www.ebi.ac.uk/pdbsum/1h8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h8s ProSAT]</span></td></tr>
-Selection, characterization and x-ray structure of anti-ampicillin single-chain Fv fragments from phage-displayed murine antibody libraries., Burmester J, Spinelli S, Pugliese L, Krebber A, Honegger A, Jung S, Schimmele B, Cambillau C, Pluckthun A, J Mol Biol. 2001 Jun 8;309(3):671-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11397088 11397088]
+</table>
-[[Category: Mouse e 010090]]
+== Evolutionary Conservation ==
-[[Category: Single protein]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Burmester, J.]]
+Check<jmol>
-[[Category: Cambillau, C.]]
+  <jmolCheckbox>
-[[Category: Honegger, A.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/1h8s_consurf.spt"</scriptWhenChecked>
-[[Category: Jung, S.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Krebber, A.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Pluckthun, A.]]
+  </jmolCheckbox>
-[[Category: Pugliese, L.]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h8s ConSurf].
-[[Category: Schimmele, B.]]
+<div style="clear:both"></div>
-[[Category: Spinelli, S.]]
+__TOC__
-[[Category: AIC]]
+</StructureSection>
-[[Category: SO4]]
+[[Category: Large Structures]]
-[[Category: anti-ampicillin antibodies]]
+[[Category: Mus musculus]]
+[[Category: Burmester J]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:11:18 2007''
+[[Category: Cambillau C]]
+[[Category: Honegger A]]
+[[Category: Jung S]]
+[[Category: Krebber A]]
+[[Category: Pluckthun A]]
+[[Category: Pugliese L]]
+[[Category: Schimmele B]]
+[[Category: Spinelli S]]

1h8s

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Current revision

Three-dimensional structure of anti-ampicillin single chain Fv fragment complexed with the hapten.

Structural highlights

Evolutionary Conservation

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