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1h8v

From Proteopedia

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[[Image:1h8v.jpg|left|200px]]
 
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{{Structure
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==The X-ray Crystal Structure of the Trichoderma reesei Family 12 Endoglucanase 3, Cel12A, at 1.9 A Resolution==
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|PDB= 1h8v |SIZE=350|CAPTION= <scene name='initialview01'>1h8v</scene>, resolution 1.90&Aring;
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<StructureSection load='1h8v' size='340' side='right'caption='[[1h8v]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE= <scene name='pdbsite=CA1:The+Active-Site+Residues+That+Are+Involve+In+Catalysis+A+...'>CA1</scene>, <scene name='pdbsite=CA2:The+Active-Site+Residues+That+Are+Involve+In+Catalysis+A+...'>CA2</scene>, <scene name='pdbsite=CA3:The+Active-Site+Residues+That+Are+Involve+In+Catalysis+A+...'>CA3</scene>, <scene name='pdbsite=CA4:The+Active-Site+Residues+That+Are+Involve+In+Catalysis+A+...'>CA4</scene>, <scene name='pdbsite=CA5:The+Active-Site+Residues+That+Are+Involve+In+Catalysis+A+...'>CA5</scene> and <scene name='pdbsite=CA6:The+Active-Site+Residues+That+Are+Involve+In+Catalysis+A+...'>CA6</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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<table><tr><td colspan='2'>[[1h8v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H8V FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h8v OCA], [https://pdbe.org/1h8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h8v RCSB], [https://www.ebi.ac.uk/pdbsum/1h8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h8v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O00095_HYPJE O00095_HYPJE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/1h8v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h8v ConSurf].
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<div style="clear:both"></div>
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'''THE X-RAY CRYSTAL STRUCTURE OF THE TRICHODERMA REESEI FAMILY 12 ENDOGLUCANASE 3, CEL12A, AT 1.9 A RESOLUTION'''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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We present the three-dimensional structure of Trichoderma reesei endoglucanase 3 (Cel12A), a small, 218 amino acid residue (24.5 kDa), neutral pI, glycoside hydrolase family 12 cellulase that lacks a cellulose-binding module. The structure has been determined using X-ray crystallography and refined to 1.9 A resolution. The asymmetric unit consists of six non-crystallographic symmetry-related molecules that were exploited to improve initial multiple isomorphous replacement phasing, and subsequent structure refinement. The enzyme contains one disulfide bridge and is glycosylated at Asp164 by a single N-acetyl glucosamine residue. The protein has the expected fold for a glycoside hydrolase clan-C family 12 enzyme. It contains two beta-sheets, of six and nine strands, packed on top of one another, and one alpha-helix. The concave surface of the nine-stranded beta-sheet forms a large substrate-binding groove in which the active-site residues are located. In the active site, we find a carboxylic acid trio, similar to that of glycoside hydrolase families 7 and 16. The strictly conserved Asp99 hydrogen bonds to the nucleophile, the invariant Glu116. The binding crevice is lined with both aromatic and polar amino acid side-chains which may play a role in substrate binding. The structure of the fungal family 12 enzyme presented here allows a complete structural characterization of the glycoside hydrolase-C clan.
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[[Category: Large Structures]]
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[[Category: Trichoderma reesei]]
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==About this Structure==
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[[Category: Bott R]]
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1H8V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8V OCA].
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[[Category: Cameron AD]]
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[[Category: Jones TA]]
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==Reference==
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[[Category: Mitchinson C]]
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The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution., Sandgren M, Shaw A, Ropp TH, Wu S, Bott R, Cameron AD, Stahlberg J, Mitchinson C, Jones TA, J Mol Biol. 2001 Apr 27;308(2):295-310. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11327768 11327768]
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[[Category: Ropp TH]]
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[[Category: Cellulase]]
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[[Category: Sandgren M]]
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[[Category: Hypocrea jecorina]]
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[[Category: Shaw A]]
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[[Category: Single protein]]
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[[Category: Stahlberg J]]
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[[Category: Bott, R.]]
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[[Category: Wu S]]
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[[Category: Cameron, A D.]]
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[[Category: Jones, T A.]]
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[[Category: Mitchinson, C.]]
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[[Category: Ropp, T H.]]
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[[Category: Sandgren, M.]]
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[[Category: Shaw, A.]]
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[[Category: Stahlberg, J.]]
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[[Category: Wu, S.]]
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[[Category: NAG]]
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[[Category: cellulase]]
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[[Category: cellulose degradation]]
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[[Category: endoglucanase]]
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[[Category: gh family 12]]
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[[Category: glycosyl hydrolase]]
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[[Category: hydrolase]]
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[[Category: trichoderma reesei cel12a]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:33:50 2008''
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Current revision

The X-ray Crystal Structure of the Trichoderma reesei Family 12 Endoglucanase 3, Cel12A, at 1.9 A Resolution

PDB ID 1h8v

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