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1h91

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[[Image:1h91.gif|left|200px]]<br />
 
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<applet load="1h91" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h91, resolution 1.40&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF LOBSTER APOCRUSTACYANIN A1 USING SOFTER X-RAYS.'''<br />
 
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==Overview==
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==The crystal structure of lobster apocrustacyanin A1 using softer X-rays.==
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The molecular basis of the camouflage colouration of marine crustacea is, often provided by carotenoproteins. The blue colour of the lobster, carapace, for example, is intricately associated with a, multimacromolecular 16-mer complex of protein subunits each with a bound, astaxanthin molecule. The protein subunits of crustacyanin fall into two, distinct subfamilies, CRTC and CRTA. Here, the crystal structure solution, of the A(1) protein of the CRTC subfamily is reported. The problematic, nature of the structure solution of the CRTC proteins (both C(1) and A(1)), warranted consideration and the development of new approaches. Three, putative disulfides per protein subunit were likely to exist based on, molecular-homology modelling against known lipocalin protein structures., With two such subunits per crystallographic asymmetric unit, this direct, approach was still difficult as it involved detecting a weak signal from, these sulfurs and suggested the use of softer X-rays, combined with high, data multiplicity, as reported previously [Chayen et al. (2000), Acta, Cryst. D56, 1064-1066]. This paper now describes the structure solution of, CRTC in the form of the A(1) dimer based on use of softer X-rays (2 A, wavelength). The structure solution involved a xenon derivative with an, optimized xenon L(I) edge f" signal and a native data set. The hand of the, xenon SIROAS phases was determined by using the sulfur anomalous signal, from a high-multiplicity native data set also recorded at 2 A wavelength., For refinement, a high-resolution data set was measured at short, wavelength. All four data sets were collected at 100 K. The refined, structure to 1.4 A resolution based on 60 276 reflections has an R factor, of 17.7% and an R(free) of 22.9% (3137 reflections). The structure is that, of a typical lipocalin, being closely related to insecticyanin, to, bilin-binding protein and to retinol-binding protein. This A(1) monomer or, dimer can now be used as a search motif in the structural studies of the, oligomeric forms alpha- and beta-crustacyanins, which contain bound, astaxanthin molecules.
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<StructureSection load='1h91' size='340' side='right'caption='[[1h91]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h91]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homarus_gammarus Homarus gammarus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H91 FirstGlance]. <br>
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1H91 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homarus_gammarus Homarus gammarus] with MPD as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Sites: AC1, AC2, AC3 and AC4. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H91 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h91 OCA], [https://pdbe.org/1h91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h91 RCSB], [https://www.ebi.ac.uk/pdbsum/1h91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h91 ProSAT]</span></td></tr>
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Structure of lobster apocrustacyanin A1 using softer X-rays., Cianci M, Rizkallah PJ, Olczak A, Raftery J, Chayen NE, Zagalsky PF, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1219-29. Epub 2001, Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11526313 11526313]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CRA1_HOMGA CRA1_HOMGA] Binds the carotenoid astaxanthin (AXT) which provides the blue coloration to the carapace of the lobster.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h91_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h91 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homarus gammarus]]
[[Category: Homarus gammarus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chayen, N.E.]]
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[[Category: Chayen NE]]
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[[Category: Cianci, M.]]
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[[Category: Cianci M]]
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[[Category: Helliwell, J.R.]]
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[[Category: Helliwell JR]]
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[[Category: Olczak, A.]]
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[[Category: Olczak A]]
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[[Category: Raftery, J.]]
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[[Category: Raftery J]]
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[[Category: Rizkallah, P.J.]]
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[[Category: Rizkallah PJ]]
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[[Category: Zagalsky, P.]]
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[[Category: Zagalsky PF]]
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[[Category: MPD]]
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[[Category: apocrustacyanin]]
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[[Category: softer x-rays]]
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[[Category: sulphurs]]
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[[Category: xenon]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:24:40 2007''
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Current revision

The crystal structure of lobster apocrustacyanin A1 using softer X-rays.

PDB ID 1h91

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