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1hg8

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[[Image:1hg8.gif|left|200px]]
 
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{{Structure
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==Endopolygalacturonase from the phytopathogenic fungus Fusarium moniliforme==
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|PDB= 1hg8 |SIZE=350|CAPTION= <scene name='initialview01'>1hg8</scene>, resolution 1.73&Aring;
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<StructureSection load='1hg8' size='340' side='right'caption='[[1hg8]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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<table><tr><td colspan='2'>[[1hg8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_verticillioides Fusarium verticillioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HG8 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hg8 OCA], [https://pdbe.org/1hg8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hg8 RCSB], [https://www.ebi.ac.uk/pdbsum/1hg8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hg8 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hg8 OCA], [http://www.ebi.ac.uk/pdbsum/1hg8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hg8 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PGLR_GIBFU PGLR_GIBFU] Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
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== Evolutionary Conservation ==
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'''ENDOPOLYGALACTURONASE FROM THE PHYTOPATHOGENIC FUNGUS FUSARIUM MONILIFORME'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hg/1hg8_consurf.spt"</scriptWhenChecked>
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To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes; among them, endopolygalacturonase (PG) catalyzes the fragmentation and solubilization of homogalacturonan. Polygalacturonase-inhibiting proteins (PGIPs), found in the cell wall of many plants, counteract fungal PGs by forming specific complexes with them. We report the crystal structure at 1.73 A resolution of PG from the phytopathogenic fungus Fusarium moniliforme (FmPG). The structure of FmPG was useful to study the mode of interaction of the enzyme with PGIP-2 from Phaseolus vulgaris. Several amino acids of FmPG were mutated, and their contribution to the formation of the complex with PGIP-2 was investigated by surface plasmon resonance. The residues Lys-269 and Arg-267, located inside the active site cleft, and His-188, at the edge of the active site cleft, are critical for the formation of the complex, which is consistent with the observed competitive inhibition of the enzyme played by PGIP-2. The replacement of His-188 with a proline or the insertion of a tryptophan after position 270, variations that both occur in plant PGs, interferes with the formation of the complex. We suggest that these variations are important structural requirements of plant PGs to prevent PGIP binding.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1HG8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gibberella_moniliformis Gibberella moniliformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG8 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hg8 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein)., Federici L, Caprari C, Mattei B, Savino C, Di Matteo A, De Lorenzo G, Cervone F, Tsernoglou D, Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13425-30. Epub 2001 Oct 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11687632 11687632]
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</StructureSection>
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[[Category: Gibberella moniliformis]]
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[[Category: Fusarium verticillioides]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Caprari, C.]]
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[[Category: Caprari C]]
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[[Category: Cervone, F.]]
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[[Category: Cervone F]]
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[[Category: Federici, L.]]
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[[Category: De Lorenzo G]]
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[[Category: Lorenzo, G De.]]
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[[Category: Federici L]]
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[[Category: Mattei, B.]]
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[[Category: Mattei B]]
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[[Category: Savino, C.]]
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[[Category: Savino C]]
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[[Category: Tsernoglou, D.]]
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[[Category: Tsernoglou D]]
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[[Category: hydrolase]]
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[[Category: pectin degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:04:28 2008''
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Current revision

Endopolygalacturonase from the phytopathogenic fungus Fusarium moniliforme

PDB ID 1hg8

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