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1hik

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(New page: 200px<br /> <applet load="1hik" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hik, resolution 2.60&Aring;" /> '''INTERLEUKIN-4 (WILD...)
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[[Image:1hik.gif|left|200px]]<br />
 
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<applet load="1hik" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hik, resolution 2.60&Aring;" />
 
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'''INTERLEUKIN-4 (WILD-TYPE)'''<br />
 
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==Overview==
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==INTERLEUKIN-4 (WILD-TYPE)==
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The structure of recombinant human interleukin-4 (hIL-4) has been, determined by both NMR and X-ray diffraction methods in several, laboratories, including ours. The X-ray and NMR structures were, successfully applied for solving the X-ray crystal structure by molecular, replacement. Due to the small size of the hIL-4 molecule (129 residues), and its lack of structural diversity (4-helix bundle), this task was, especially difficult and required special care with rotation function, applications. The crucial point was that proper removal of the Patterson, origin peaks was indispensable in all cases. All available structures of, hIL-4 were checked, in a standardized procedure, for their suitability as, templates for molecular replacement. The models derived from the various, structures are close to, but not in all loop details identical with, the, genuine X-ray structures. The deviations of the X-ray structure-derived, models are of the same magnitude as the differences between the original, X-ray structures, while the deviations of the NMR structure-derived models, are two to three times as large. The hIL-4 variant R88Q is a binding, mutant, its affinity to the receptor is decreased by a factor of about, 200. Its X-ray structure was determined by molecular replacement using the, wild-type X-ray structure determined in our laboratory as a model. The, structure of R88Q is virtually identical with that of the wild-type, protein. All differences besides the shortened side-chain of residue 88, occur at surface residues with high temperature factors, i.e. at spots, where the structure is not well defined. Since the structure is not, perturbed, the biological effect of decreased receptor affinity has to be, attributed to the loss of a single positive charge in the surface area of, the main receptor contact.
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<StructureSection load='1hik' size='340' side='right'caption='[[1hik]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hik]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HIK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hik OCA], [https://pdbe.org/1hik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hik RCSB], [https://www.ebi.ac.uk/pdbsum/1hik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hik ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/IL4_HUMAN IL4_HUMAN] Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:[https://omim.org/entry/601367 601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.<ref>PMID:14681304</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/IL4_HUMAN IL4_HUMAN] Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/1hik_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hik ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known diseases associated with this structure: AIDS, slow progression to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147781 147781]], Atopy, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147781 147781]]
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*[[Interleukin 3D structures|Interleukin 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1HIK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HIK OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Human interleukin-4 and variant R88Q: phasing X-ray diffraction data by molecular replacement using X-ray and nuclear magnetic resonance models., Muller T, Oehlenschlager F, Buehner M, J Mol Biol. 1995 Mar 24;247(2):360-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7707380 7707380]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Buehner, M.]]
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[[Category: Buehner M]]
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[[Category: Mueller, T.]]
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[[Category: Mueller T]]
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[[Category: cytokine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:18:34 2007''
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Current revision

INTERLEUKIN-4 (WILD-TYPE)

PDB ID 1hik

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