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1hj9

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Atomic resolution structures of trypsin provide insight into structural radiation damage

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Retrieved from "http://52.214.119.220/wiki/index.php/1hj9"

Categories: Bos taurus | Large Structures | Leiros H-KS | McSweeney SM | Smalas AO

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-[[Image:1hj9.gif|left|200px]]<br />
-<applet load="1hj9" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1hj9, resolution 0.95&Aring;" />
-'''ATOMIC RESOLUTION STRUCTURES OF TRYPSIN PROVIDE INSIGHT INTO STRUCTURAL RADIATION DAMAGE'''<br />
-==Overview==
+==Atomic resolution structures of trypsin provide insight into structural radiation damage==
-Radiation damage is an inherent problem in protein X-ray crystallography, and the process has recently been shown to be highly specific, exhibiting, features such as cleavage of disulfide bonds, decarboxylation of acidic, residues, increase in atomic B factors and increase in unit-cell volume., Reported here are two trypsin structures at atomic resolution (1.00 and, 0.95 A), the data for which were collected at a third-generation, synchrotron (ESRF) at two different beamlines. Both trypsin structures, exhibit broken disulfide bonds; in particular, the bond from Cys191 to, Cys220 is very sensitive to synchrotron radiation. The data set collected, at the most intense beamline (ID14-EH4) shows increased structural, radiation damage in terms of lower occupancies for cysteine residues, more, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11264577 (full description)]]
+<StructureSection load='1hj9' size='340' side='right'caption='[[1hj9]], [[Resolution|resolution]] 0.95&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1hj9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJ9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.95&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANL:ANILINE'>ANL</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hj9 OCA], [https://pdbe.org/1hj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hj9 RCSB], [https://www.ebi.ac.uk/pdbsum/1hj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hj9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hj9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hj9 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-HJ9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with SO4, CA, ANL and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJ9 OCA]].
+*[[Trypsin 3D structures|Trypsin 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Atomic resolution structures of trypsin provide insight into structural radiation damage., Leiros HK, McSweeney SM, Smalas AO, Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):488-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11264577 11264577]
 [[Category: Bos taurus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Leiros, H.K.S.]]
+[[Category: Leiros H-KS]]
-[[Category: Mcsweeney, S.M.]]
+[[Category: McSweeney SM]]
-[[Category: Smalas, A.O.]]
+[[Category: Smalas AO]]
-[[Category: ANL]]
-[[Category: CA]]
-[[Category: GOL]]
-[[Category: SO4]]
-[[Category: atomic resolution]]
-[[Category: disulphid bond breakage]]
-[[Category: radiation damage]]
-[[Category: serine proteinase]]
-[[Category: trypsin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:27:58 2007''

1hj9

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Atomic resolution structures of trypsin provide insight into structural radiation damage

Structural highlights

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Evolutionary Conservation

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