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1hkl

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(New page: 200px<br /> <applet load="1hkl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hkl, resolution 2.68&Aring;" /> '''FREE AND LIGANDED F...)
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[[Image:1hkl.gif|left|200px]]<br />
 
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<applet load="1hkl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hkl, resolution 2.68&Aring;" />
 
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'''FREE AND LIGANDED FORM OF AN ESTEROLYTIC CATALYTIC ANTIBODY'''<br />
 
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==Overview==
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==FREE AND LIGANDED FORM OF AN ESTEROLYTIC CATALYTIC ANTIBODY==
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The crystal structure of the esterase catalytic antibody 48G7 has been, determined in the presence of hapten at 2.0 A resolution and in the, absence of hapten at 2.7 A resolution. The root-mean-square difference, between the two structures is 0.6 A for the variable domain and 0.7 A for, the constant domain. Comparison of the active site shows that no, significant changes occur upon hapten binding as main-chain and side-chain, displacements are negligible. Complex formation occurs as hapten fits into, a pre-formed pocket about 10 A deep. Although 151 water molecules were, modeled into the 48G7-hapten structure, none are bound in the active site., Comparison of the 48G7 structures with those of other published ester, hydrolysis antibodies illustrates an emerging theme used by esterolytic, antibodies in binding their (nitro-)phenyl haptens and in hydrolysing, their cognate esters and carbonates: hapten is bound with the aryl end, buried deep in the binding pocket, and the phosphonate moiety is, responsible for the majority of the binding energy to the antibody-hapten, interaction.
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<StructureSection load='1hkl' size='340' side='right'caption='[[1hkl]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hkl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HKL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.68&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkl OCA], [https://pdbe.org/1hkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hkl RCSB], [https://www.ebi.ac.uk/pdbsum/1hkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hkl ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/IGKC_HUMAN IGKC_HUMAN] Defects in IGKC are the cause of immunoglobulin kappa light chain deficiency (IGKCD) [MIM:[https://omim.org/entry/614102 614102]. IGKCD is a disease characterized by the complete absence of immunoglobulin kappa chains.<ref>PMID:3931219</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/IGKC_HUMAN IGKC_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hkl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hkl ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known disease associated with this structure: Kappa light chain deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147200 147200]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1HKL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HKL OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Crystal structures of the free and liganded form of an esterolytic catalytic antibody., Wedemayer GJ, Wang LH, Patten PA, Schultz PG, Stevens RC, J Mol Biol. 1997 May 2;268(2):390-400. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9159478 9159478]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Patten, P.A.]]
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[[Category: Patten PA]]
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[[Category: Schultz, P.G.]]
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[[Category: Schultz PG]]
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[[Category: Stevens, R.C.]]
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[[Category: Stevens RC]]
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[[Category: Wang, L.H.]]
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[[Category: Wang LH]]
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[[Category: Wedemayer, G.J.]]
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[[Category: Wedemayer GJ]]
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[[Category: catalytic antibody]]
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[[Category: ester hydrolysis]]
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[[Category: esterolytic]]
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[[Category: fab]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:19:30 2007''
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Current revision

FREE AND LIGANDED FORM OF AN ESTEROLYTIC CATALYTIC ANTIBODY

PDB ID 1hkl

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