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1hku

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[[Image:1hku.gif|left|200px]]
 
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{{Structure
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==CtBP/BARS: a dual-function protein involved in transcription corepression and Golgi membrane fission==
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|PDB= 1hku |SIZE=350|CAPTION= <scene name='initialview01'>1hku</scene>, resolution 2.3&Aring;
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<StructureSection load='1hku' size='340' side='right'caption='[[1hku]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Nad+Binding+Site+For+Chain+A'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
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<table><tr><td colspan='2'>[[1hku]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HKU FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hku OCA], [https://pdbe.org/1hku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hku RCSB], [https://www.ebi.ac.uk/pdbsum/1hku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hku ProSAT]</span></td></tr>
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</table>
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'''CTBP/BARS: A DUAL-FUNCTION PROTEIN INVOLVED IN TRANSCRIPTION COREPRESSION AND GOLGI MEMBRANE FISSION'''
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== Function ==
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[https://www.uniprot.org/uniprot/CTBP1_RAT CTBP1_RAT] Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity. Functions in brown adipose tissue (BAT) differentiation.<ref>PMID:10364211</ref> <ref>PMID:7624370</ref> <ref>PMID:12805226</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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C-terminal-binding protein/brefeldin A-ADP ribosylated substrate (CtBP/BARS) plays key roles in development and oncogenesis as a transcription co-repressor, and in intracellular traffic as a promoter of Golgi membrane fission. Co-repressor activity is regulated by NAD(H) binding to CtBP/BARS, while membrane fission is associated with its acyl-CoA-dependent acyltransferase activity. Here, we report the crystal structures of rat CtBP/BARS in a binary complex with NAD(H), and in a ternary complex with a PIDLSKK peptide mimicking the consensus motif (PXDLS) recognized in CtBP/BARS cellular partners. The structural data show CtBP/BARS in a NAD(H)-bound dimeric form; the peptide binding maps the recognition site for DNA-binding proteins and histone deacetylases to an N-terminal region of the protein. The crystal structure together with the site-directed mutagenesis data and binding experiments suggest a rationale for the molecular mechanisms underlying the two fundamental co-existing, but diverse, activities supported by CtBP/BARS in the nucleus and in Golgi membranes.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hku_consurf.spt"</scriptWhenChecked>
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1HKU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKU OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission., Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M, EMBO J. 2003 Jun 16;22(12):3122-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12805226 12805226]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hku ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Bolognesi M]]
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[[Category: Bolognesi, M.]]
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[[Category: Cericola C]]
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[[Category: Cericola, C.]]
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[[Category: Corda D]]
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[[Category: Corda, D.]]
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[[Category: Luini A]]
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[[Category: Luini, A.]]
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[[Category: Massaro A]]
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[[Category: Massaro, A.]]
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[[Category: Millo E]]
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[[Category: Millo, E.]]
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[[Category: Nardini M]]
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[[Category: Nardini, M.]]
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[[Category: Pesce A]]
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[[Category: Pesce, A.]]
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[[Category: Spano S]]
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[[Category: Spano , S.]]
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[[Category: FMT]]
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[[Category: GOL]]
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[[Category: NAD]]
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[[Category: acyl-coa]]
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[[Category: acyltransferase]]
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[[Category: brefeldin some]]
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[[Category: golgi membrane]]
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[[Category: nad]]
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[[Category: transcription co-repression]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:38:24 2008''
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Current revision

CtBP/BARS: a dual-function protein involved in transcription corepression and Golgi membrane fission

PDB ID 1hku

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