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1hro

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(New page: 200px<br /><applet load="1hro" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hro, resolution 2.2&Aring;" /> '''MOLECULAR STRUCTURE O...)
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[[Image:1hro.gif|left|200px]]<br /><applet load="1hro" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hro, resolution 2.2&Aring;" />
 
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'''MOLECULAR STRUCTURE OF A HIGH POTENTIAL CYTOCHROME C2 ISOLATED FROM RHODOPILA GLOBIFORMIS'''<br />
 
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==Overview==
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==MOLECULAR STRUCTURE OF A HIGH POTENTIAL CYTOCHROME C2 ISOLATED FROM RHODOPILA GLOBIFORMIS==
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Unlike their mitochondrial counterparts, the c-type cytochromes typically, isolated from photosynthetic nonsulfur purple bacteria display a wide, range of oxidation-reduction potentials. Here we describe the X-ray, crystallographic analysis of the cytochrome c2 isolated from Rhodopila, globiformis. This particular c-type cytochrome was selected for study, because of its anomalously high redox potential of +450 mV. Crystals, employed in the investigation belonged to the space group I4(1) with unit, cell dimensions of a = b = 79.2 A, c = 75.2 A, and two molecules in the, asymmetric unit. The structure was solved by the techniques of multiple, isomorphous replacement with two heavy-atom derivatives and electron, density modification procedures. Least-squares refinement of the model, reduced the R-factor to 18.7% for all measured X-ray data from 30.0 to 2.2, A. The overall structural motif of the protein is composed of five, alpha-helices, one type I turn, and six type II turns. As in other, cytochromes c, there are two conserved water molecules located in the, heme-binding pocket. Overall, the three-dimensional structure of the R., globiformis molecule is more similar to the eukaryotic c-type cytochromes, than to other bacterial proteins.
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<StructureSection load='1hro' size='340' side='right'caption='[[1hro]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodopila_globiformis Rhodopila globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HRO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hro OCA], [https://pdbe.org/1hro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hro RCSB], [https://www.ebi.ac.uk/pdbsum/1hro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hro ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYC2_RHOGL CYC2_RHOGL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/1hro_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hro ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1HRO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodopila_globiformis Rhodopila globiformis] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HRO OCA].
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*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Molecular structure of a high potential cytochrome c2 isolated from Rhodopila globiformis., Benning MM, Meyer TE, Holden HM, Arch Biochem Biophys. 1996 Sep 15;333(2):338-48. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8809072 8809072]
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[[Category: Large Structures]]
[[Category: Rhodopila globiformis]]
[[Category: Rhodopila globiformis]]
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[[Category: Single protein]]
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[[Category: Benning MM]]
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[[Category: Benning, M.M.]]
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[[Category: Holden HM]]
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[[Category: Holden, H.M.]]
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[[Category: Meyer TE]]
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[[Category: Meyer, T.E.]]
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[[Category: HEM]]
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[[Category: electron transport]]
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[[Category: heme]]
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[[Category: photosynthesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:47:11 2007''
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Current revision

MOLECULAR STRUCTURE OF A HIGH POTENTIAL CYTOCHROME C2 ISOLATED FROM RHODOPILA GLOBIFORMIS

PDB ID 1hro

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