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1hsr

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[[Image:1hsr.gif|left|200px]]<br />
 
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<applet load="1hsr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hsr, resolution 1.60&Aring;" />
 
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'''BINDING MODE OF BENZHYDROXAMIC ACID TO ARTHROMYCES RAMOSUS PEROXIDASE'''<br />
 
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==Overview==
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==BINDING MODE OF BENZHYDROXAMIC ACID TO ARTHROMYCES RAMOSUS PEROXIDASE==
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The crystal structure of Arthromyces ramosus peroxidase (ARP) in complex, with benzhydroxamic acid (BHA) as determined by X-ray analysis at 1.6 A, shows unambiguously how BHA binds to ARP. BHA is located in the distal, heme pocket. Its functional groups are held by three hydrogen bonds to, His56N(epsilon), Arg52N(epsilon), and Pro(154)O, but are too far away to, interact with the heme iron. The aromatic ring of BHA is positioned at the, entrance of the channel to the heme pocket, approximately parallel to the, heme group. Most water molecules at the active site of the native enzyme, are replaced by BHA, leaving a ligand, probably a water molecule, at the, sixth position of the heme. Results are compared with spectroscopic data.
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<StructureSection load='1hsr' size='340' side='right'caption='[[1hsr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1hsr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricales_sp._'Arthromyces_ramosus' Agaricales sp. 'Arthromyces ramosus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HSR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HSR FirstGlance]. <br>
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1HSR is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Eukaryota Eukaryota]] with CA, HEM and BHO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Peroxidase Peroxidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7]]. Structure known Active Sites: CA1, CA2 and GL1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HSR OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BHO:BENZHYDROXAMIC+ACID'>BHO</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hsr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hsr OCA], [https://pdbe.org/1hsr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hsr RCSB], [https://www.ebi.ac.uk/pdbsum/1hsr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hsr ProSAT]</span></td></tr>
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Binding mode of benzhydroxamic acid to Arthromyces ramosus peroxidase shown by X-ray crystallographic analysis of the complex at 1.6 A resolution., Itakura H, Oda Y, Fukuyama K, FEBS Lett. 1997 Jul 21;412(1):107-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9257700 9257700]
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</table>
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[[Category: Eukaryota]]
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== Function ==
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[[Category: Peroxidase]]
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[https://www.uniprot.org/uniprot/PER_ARTRA PER_ARTRA]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Fukuyama, K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Itakura, H.]]
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Check<jmol>
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[[Category: BHO]]
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<jmolCheckbox>
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[[Category: CA]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hs/1hsr_consurf.spt"</scriptWhenChecked>
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[[Category: HEM]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: glycoprotein]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: oxidoreductase]]
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</jmolCheckbox>
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[[Category: peroxidase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hsr ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:07:39 2007''
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__TOC__
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</StructureSection>
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[[Category: Agaricales sp. 'Arthromyces ramosus']]
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[[Category: Large Structures]]
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[[Category: Fukuyama K]]
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[[Category: Itakura H]]

Current revision

BINDING MODE OF BENZHYDROXAMIC ACID TO ARTHROMYCES RAMOSUS PEROXIDASE

PDB ID 1hsr

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