1a6c

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[[Image:1a6c.gif|left|200px]]<br /><applet load="1a6c" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a6c, resolution 3.5&Aring;" />
 
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'''STRUCTURE OF TOBACCO RINGSPOT VIRUS'''<br />
 
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==Overview==
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==STRUCTURE OF TOBACCO RINGSPOT VIRUS==
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BACKGROUND: Tobacco ringspot virus (TRSV) is a member of the nepovirus genus of icosahedral RNA plant viruses that cause disease in fruit crops. Nepoviruses, comoviruses and picornaviruses are classified in the picornavirus superfamily. Crystal structures of comoviruses and picornaviruses and the molecular mass of the TRSV subunit (sufficient to accommodate three beta-barrel domains) suggested that nepoviruses may represent a link in the evolution of the picornavirus capsids from a T = 3 icosahedral virus. This evolutionary process is thought to involve triplication of the capsid protein gene, to encode a three-domain polyprotein, followed by development of cleavage sites in the interdomain linking regions. Structural studies on TRSV were initiated to determine if the TRSV subunit corresponds to the proposed uncleaved three-domain polyprotein. RESULTS: The 3.5 A resolution structure of TRSV shows that the capsid protein consists of three beta-barrel domains covalently linked by extended polypeptides. The order of connectivity of the domains in TRSV confirms the proposed connectivity for the precleaved comovirus and picornavirus capsid polyprotein. Structural differences between equivalent domains in TRSV and comoviruses are confined to the external surface loops, interdomain connecting polypeptides and N termini. The three different domains within TRSV and comoviruses are more closely related at the structural level than the three individual domains within picornaviruses. CONCLUSIONS: The structural results confirm the notion of divergent evolution of the capsid polyproteins of nepoviruses, comoviruses and picornaviruses from a common ancestor. A number of residues were found to be conserved among various nepoviruses, some of which stabilize the quaternary structure of the three domains in the TRSV capsid protein subunit. Two conserved regions were identified on the external surface of TRSV, however, mutational studies will be needed to understand their functional significance. Nepoviruses transmitted by the same nematode species do not share regions with similar amino acid composition on the viral surface.
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<StructureSection load='1a6c' size='340' side='right'caption='[[1a6c]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a6c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_ringspot_virus Tobacco ringspot virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A6C FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a6c OCA], [https://pdbe.org/1a6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a6c RCSB], [https://www.ebi.ac.uk/pdbsum/1a6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a6c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_TRSV CAPSD_TRSV]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a6c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a6c ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1A6C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tobacco_ringspot_virus Tobacco ringspot virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A6C OCA].
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The structure of tobacco ringspot virus: a link in the evolution of icosahedral capsids in the picornavirus superfamily., Chandrasekar V, Johnson JE, Structure. 1998 Feb 15;6(2):157-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9519407 9519407]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Tobacco ringspot virus]]
[[Category: Tobacco ringspot virus]]
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[[Category: Chandrasekar, V.]]
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[[Category: Chandrasekar V]]
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[[Category: Johnson, J E.]]
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[[Category: Johnson JE]]
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[[Category: icosahedral virus]]
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[[Category: nepovirus]]
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[[Category: picornavirus superfamily]]
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[[Category: trsv]]
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[[Category: virus capsid protein]]
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[[Category: virus evolution]]
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[[Category: virus structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:24 2008''
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Current revision

STRUCTURE OF TOBACCO RINGSPOT VIRUS

PDB ID 1a6c

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