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1coi

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(New page: 200px<br /><applet load="1coi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1coi, resolution 2.1&Aring;" /> '''DESIGNED TRIMERIC COI...)
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[[Image:1coi.jpg|left|200px]]<br /><applet load="1coi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1coi, resolution 2.1&Aring;" />
 
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'''DESIGNED TRIMERIC COILED COIL-VALD'''<br />
 
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==Overview==
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==DESIGNED TRIMERIC COILED COIL-VALD==
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The three-dimensional structure of the 29-residue designed coiled coil, having the amino acid sequence acetyl-E VEALEKK VAALESK VQALEKK, VEALEHG-amide has been determined and refined to a crystallographic, R-factor of 21.4% for all data from 10-A to 2.1-A resolution. This, molecule is called coil-VaLd because it contains valine in the a heptad, positions and leucine in the d heptad positions. In the trigonal crystal, three molecules, related by a crystallographic threefold axis, form a, parallel three-helix bundle. The bundles are stacked head-to-tail to form, a continuous coiled coil along the c-direction of the crystal. The, contacts among the three helices within the coiled coil are mainly, hydrophobic: four layers of valine residues alternate with four layers of, leucine residues to form the core of the bundle. In contrast, mostly, hydrophilic contacts mediate the interaction between trimers: here a total, of two direct protein--protein hydrogen bonds are found. Based on the, structure, we propose a scheme for designing crystals of peptides, containing continuous two-, three-, and four-stranded coiled coils.
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<StructureSection load='1coi' size='340' side='right'caption='[[1coi]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1coi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1COI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1COI FirstGlance]. <br>
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1COI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct] with SO4, ACE and NH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1COI OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1coi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1coi OCA], [https://pdbe.org/1coi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1coi RCSB], [https://www.ebi.ac.uk/pdbsum/1coi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1coi ProSAT]</span></td></tr>
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The crystal structure of the designed trimeric coiled coil coil-VaLd: implications for engineering crystals and supramolecular assemblies., Ogihara NL, Weiss MS, Degrado WF, Eisenberg D, Protein Sci. 1997 Jan;6(1):80-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9007979 9007979]
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</table>
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[[Category: Protein complex]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Synthetic construct]]
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[[Category: Degrado, W.F.]]
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[[Category: Degrado WF]]
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[[Category: Eisenberg, D.]]
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[[Category: Eisenberg D]]
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[[Category: Ogihara, N.L.]]
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[[Category: Ogihara NL]]
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[[Category: Weiss, M.S.]]
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[[Category: Weiss MS]]
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[[Category: ACE]]
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[[Category: NH2]]
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[[Category: SO4]]
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[[Category: alpha-helical bundle]]
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[[Category: coiled coil design]]
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[[Category: protein design]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:44:58 2007''
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Current revision

DESIGNED TRIMERIC COILED COIL-VALD

PDB ID 1coi

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