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1jrg

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(New page: 200px<br /><applet load="1jrg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jrg, resolution 2.1&Aring;" /> '''Crystal Structure of ...)
Current revision (06:04, 3 April 2024) (edit) (undo)
 
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[[Image:1jrg.jpg|left|200px]]<br /><applet load="1jrg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jrg, resolution 2.1&Aring;" />
 
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'''Crystal Structure of the R3 form of Pectate Lyase A, Erwinia chrysanthemi'''<br />
 
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==Overview==
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==Crystal Structure of the R3 form of Pectate Lyase A, Erwinia chrysanthemi==
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Pectate lyase A is a virulence factor secreted by the plant-pathogenic, bacteria Erwinia chrysanthemi. The enzyme cleaves the glycosidic bond of, pectate polymers by a calcium-dependent beta-elimination mechanism. The, crystal structure of pectate lyase A from E. chrysanthemi EC16 has been, determined in two crystal forms, monoclinic C2 to 1.8 A and rhombohedral, R3 to 2.1 A. The protein structure is compared with two other pectate, lyase isoforms from E. chrysanthemi EC16, pectate lyase C and pectate, lyase E. Pectate lyase A is unique as it is the only acidic pectate lyase, and has end products that are significantly more varied in length in, comparison to those of the other four major pectate lyase isozymes., Differences and similarities in polypeptide trace, size and volume of the, active-site groove and surface electrostatics are discussed.
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<StructureSection load='1jrg' size='340' side='right'caption='[[1jrg]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jrg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JRG FirstGlance]. <br>
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1JRG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JRG OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jrg OCA], [https://pdbe.org/1jrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jrg RCSB], [https://www.ebi.ac.uk/pdbsum/1jrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jrg ProSAT]</span></td></tr>
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Structure of pectate lyase A: comparison to other isoforms., Thomas LM, Doan CN, Oliver RL, Yoder MD, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):1008-15. Epub, 2002 May 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12037303 12037303]
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</table>
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[[Category: Erwinia chrysanthemi]]
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== Function ==
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[[Category: Pectate lyase]]
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[https://www.uniprot.org/uniprot/PLYA_DICCH PLYA_DICCH] Involved in maceration and soft-rotting of plant tissue.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Doan, C.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Oliver, R.L.]]
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Check<jmol>
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[[Category: Thomas, L.M.]]
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<jmolCheckbox>
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[[Category: Yoder, M.D.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jr/1jrg_consurf.spt"</scriptWhenChecked>
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[[Category: SO4]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: parallel beta helix beta elimination]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:31:11 2007''
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jrg ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Dickeya chrysanthemi]]
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[[Category: Large Structures]]
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[[Category: Doan C]]
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[[Category: Oliver RL]]
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[[Category: Thomas LM]]
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[[Category: Yoder MD]]

Current revision

Crystal Structure of the R3 form of Pectate Lyase A, Erwinia chrysanthemi

PDB ID 1jrg

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