This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1jta

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1jta" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jta, resolution 1.8&Aring;" /> '''Crystal Structure of ...)
Current revision (06:04, 3 April 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1jta.gif|left|200px]]<br /><applet load="1jta" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1jta, resolution 1.8&Aring;" />
 
-
'''Crystal Structure of Pectate Lyase A (C2 form)'''<br />
 
-
==Overview==
+
==Crystal Structure of Pectate Lyase A (C2 form)==
-
Pectate lyase A is a virulence factor secreted by the plant-pathogenic, bacteria Erwinia chrysanthemi. The enzyme cleaves the glycosidic bond of, pectate polymers by a calcium-dependent beta-elimination mechanism. The, crystal structure of pectate lyase A from E. chrysanthemi EC16 has been, determined in two crystal forms, monoclinic C2 to 1.8 A and rhombohedral, R3 to 2.1 A. The protein structure is compared with two other pectate, lyase isoforms from E. chrysanthemi EC16, pectate lyase C and pectate, lyase E. Pectate lyase A is unique as it is the only acidic pectate lyase, and has end products that are significantly more varied in length in, comparison to those of the other four major pectate lyase isozymes., Differences and similarities in polypeptide trace, size and volume of the, active-site groove and surface electrostatics are discussed.
+
<StructureSection load='1jta' size='340' side='right'caption='[[1jta]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1jta]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JTA FirstGlance]. <br>
-
1JTA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pectate_lyase Pectate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.2 4.2.2.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JTA OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jta OCA], [https://pdbe.org/1jta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jta RCSB], [https://www.ebi.ac.uk/pdbsum/1jta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jta ProSAT]</span></td></tr>
-
Structure of pectate lyase A: comparison to other isoforms., Thomas LM, Doan CN, Oliver RL, Yoder MD, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):1008-15. Epub, 2002 May 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12037303 12037303]
+
</table>
-
[[Category: Erwinia chrysanthemi]]
+
== Function ==
-
[[Category: Pectate lyase]]
+
[https://www.uniprot.org/uniprot/PLYA_DICCH PLYA_DICCH] Involved in maceration and soft-rotting of plant tissue.
-
[[Category: Single protein]]
+
== Evolutionary Conservation ==
-
[[Category: Doan, C.N.]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: Oliver, R.L.]]
+
Check<jmol>
-
[[Category: Thomas, L.M.]]
+
<jmolCheckbox>
-
[[Category: Yoder, M.D.]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jta_consurf.spt"</scriptWhenChecked>
-
[[Category: SO4]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
[[Category: parallel beta helix beta-elimination mechanism]]
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:33:38 2007''
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jta ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Dickeya chrysanthemi]]
 +
[[Category: Large Structures]]
 +
[[Category: Doan CN]]
 +
[[Category: Oliver RL]]
 +
[[Category: Thomas LM]]
 +
[[Category: Yoder MD]]

Current revision

Crystal Structure of Pectate Lyase A (C2 form)

PDB ID 1jta

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jta"
Personal tools