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1n90

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[[Image:1n90.gif|left|200px]]<br />
 
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<applet load="1n90" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n90, resolution 2.90&Aring;" />
 
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'''FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA'''<br />
 
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==Overview==
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==FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA==
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The structures of nitrite reductase from Paracoccus denitrificans GB17, (NiR-Pd) and Pseudomonas aeruginosa (NiR-Pa) have been described for the, oxidized and reduced state (Fulop, V., Moir, J. W. B., Ferguson, S. J., and Hajdu, J. (1995) Cell 81, 369-377; Nurizzo, D., Silvestrini, M. C., Mathieu, M., Cutruzzola, F., Bourgeois, D., Fulop, V., Hajdu, J., Brunori, M., Tegoni, M., and Cambillau, C. (1997) Structure 5, 1157-1171; Nurizzo, D., Cutruzzola, F., Arese, M., Bourgeois, D., Brunori, M., Cambillau, C. , and Tegoni, M. (1998) Biochemistry 37, 13987-13996). Major conformational, rearrangements are observed in the extreme states although they are more, substantial in NiR-Pd. The four structures differ significantly in the c, heme domains. Upon reduction, a His17/Met106 heme-ligand ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10329702 (full description)]]
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<StructureSection load='1n90' size='340' side='right'caption='[[1n90]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n90]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N90 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHE:HEME+D'>DHE</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n90 OCA], [https://pdbe.org/1n90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n90 RCSB], [https://www.ebi.ac.uk/pdbsum/1n90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n90 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NIRS_PSEAE NIRS_PSEAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/1n90_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n90 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N90 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with HEC and DHE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.2 1.9.3.2]]. Structure known Active Sites: NIA and NIB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N90 OCA]].
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*[[Cytochrome c nitrite reductase|Cytochrome c nitrite reductase]]
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__TOC__
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==Reference==
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</StructureSection>
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Does the reduction of c heme trigger the conformational change of crystalline nitrite reductase?, Nurizzo D, Cutruzzola F, Arese M, Bourgeois D, Brunori M, Cambillau C, Tegoni M, J Biol Chem. 1999 May 21;274(21):14997-5004. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10329702 10329702]
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Cambillau C]]
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[[Category: Transferred entry: 1.7.2.1]]
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[[Category: Nurizzo D]]
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[[Category: Cambillau, C.]]
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[[Category: Tegoni M]]
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[[Category: Nurizzo, D.]]
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[[Category: Tegoni, M.]]
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[[Category: DHE]]
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[[Category: HEC]]
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[[Category: conformational change]]
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[[Category: denitrification]]
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[[Category: hemoprotein]]
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[[Category: microspectrophotometry]]
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[[Category: nitrite reductase]]
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[[Category: oxidoreductase]]
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[[Category: pseudomonas aeruginosa]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:36:10 2007''
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Current revision

FOLLOWING THE C HEME REDUCTION IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA

PDB ID 1n90

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