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1nno

From Proteopedia

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(New page: 200px<br /> <applet load="1nno" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nno, resolution 2.65&Aring;" /> '''CONFORMATIONAL CHAN...)
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[[Image:1nno.gif|left|200px]]<br />
 
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<applet load="1nno" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nno, resolution 2.65&Aring;" />
 
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'''CONFORMATIONAL CHANGES OCCURRING UPON NO BINDING IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA'''<br />
 
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==Overview==
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==CONFORMATIONAL CHANGES OCCURRING UPON NO BINDING IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA==
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Nitrite reductase (NiR) from Pseudomonas aeruginosa (EC 1.9.3.2) (NiR-Pa), is a soluble enzyme catalyzing the reduction of nitrite (NO2-) to nitric, oxide (NO). The enzyme is a 120 kDa homodimer, in which each monomer, carries one c and one d1 heme. The oxidized and reduced forms of NiR from, Paracoccus denitrificans GB17 (previously called Thiosphaera pantotropha), (NiR-Pd) have been described [Fulop, V., et al. (1995) Cell 81, 369-377;, Williams, P. A., et al. (1997) Nature 389, 406-412], and we recently, reported on the structure of oxidized NiR-Pa at 2.15 A [Nurizzo, D., et, al. (1997) Structure 5, 1157-1171]. Although the domains carrying the d1, heme are almost identical in both NiR-Pa and NiR-Pd oxidized and reduced, structures, the c heme domains show a different pattern of c ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9760233 (full description)]]
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<StructureSection load='1nno' size='340' side='right'caption='[[1nno]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nno]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NNO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHE:HEME+D'>DHE</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nno OCA], [https://pdbe.org/1nno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nno RCSB], [https://www.ebi.ac.uk/pdbsum/1nno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nno ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NIRS_PSEAE NIRS_PSEAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/1nno_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nno ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NNO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with HEC, DHE and NO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.2 1.9.3.2]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NNO OCA]].
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*[[Cytochrome c nitrite reductase|Cytochrome c nitrite reductase]]
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__TOC__
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==Reference==
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</StructureSection>
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Conformational changes occurring upon reduction and NO binding in nitrite reductase from Pseudomonas aeruginosa., Nurizzo D, Cutruzzola F, Arese M, Bourgeois D, Brunori M, Cambillau C, Tegoni M, Biochemistry. 1998 Oct 6;37(40):13987-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9760233 9760233]
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Cambillau C]]
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[[Category: Cambillau, C.]]
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[[Category: Nurizzo D]]
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[[Category: Nurizzo, D.]]
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[[Category: Tegoni M]]
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[[Category: Tegoni, M.]]
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[[Category: DHE]]
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[[Category: HEC]]
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[[Category: NO]]
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[[Category: conformational changes]]
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[[Category: denitrification]]
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[[Category: hemoprotein]]
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[[Category: nitrite reductase]]
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[[Category: no binding]]
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[[Category: oxidoreductase]]
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[[Category: pseudomonas aeruginosa]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:41:19 2007''
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Current revision

CONFORMATIONAL CHANGES OCCURRING UPON NO BINDING IN NITRITE REDUCTASE FROM PSEUDOMONAS AERUGINOSA

PDB ID 1nno

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