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1ow4

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[[Image:1ow4.gif|left|200px]]
 
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{{Structure
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==Crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with the fluorescent reporter ANS (1-anilinonaphtalene-8-sulfonic acid),==
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|PDB= 1ow4 |SIZE=350|CAPTION= <scene name='initialview01'>1ow4</scene>, resolution 1.60&Aring;
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<StructureSection load='1ow4' size='340' side='right'caption='[[1ow4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=2AN:1-ANILINO-8-NAPHTHALENE+SULFONATE'>2AN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[1ow4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhyparobia_maderae Rhyparobia maderae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OW4 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE= Pheromone Binding Protein (PBP) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6988 Leucophaea maderae])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AN:8-ANILINO-1-NAPHTHALENE+SULFONATE'>2AN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow4 OCA], [https://pdbe.org/1ow4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ow4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ow4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ow4 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1org|1ORG]], [[1p28|1P28]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ow4 OCA], [http://www.ebi.ac.uk/pdbsum/1ow4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ow4 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q8MTC1_RHYMA Q8MTC1_RHYMA]
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== Evolutionary Conservation ==
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'''Crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with the fluorescent reporter ANS (1-anilinonaphtalene-8-sulfonic acid),'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/1ow4_consurf.spt"</scriptWhenChecked>
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Pheromone-binding proteins (PBPs) are small helical proteins found in sensorial organs, particularly in the antennae, of moth and other insect species. They were proposed to solubilize and carry the hydrophobic pheromonal compounds through the antennal lymph to receptors, participating thus in the peri-receptor events of signal transduction. The x-ray structure of Bombyx mori PBP (BmorPBP), from male antennae, revealed a six-helix fold forming a cavity that contains the pheromone bombykol. We have identified a PBP (LmaPBP) from the cockroach Leucophaea maderae in the antennae of the females, the gender attracted by pheromones in this species. Here we report the crystal structure of LmaPBP alone or in complex with a fluorescent reporter (amino-naphthalen sulfonate, ANS) or with a component of the pheromonal blend, 3-hydroxy-butan-2-one. Both compounds bind in the internal cavity of LmaPBP, which is more hydrophilic than BmorPBP cavity. LmaPBP structure ends just after the sixth helix (helix F). BmorPBP structure extends beyond the sixth helix with a stretch of residues elongated at neutral pH and folding as a seventh internalized helix at low pH. These differences between LmaPBP and BmorPBP structures suggest that different binding and release mechanism may be adapted to the hydrophilicity or hydrophobicity of the pheromonal ligand.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1OW4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leucophaea_maderae Leucophaea maderae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OW4 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ow4 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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The crystal structure of a cockroach pheromone-binding protein suggests a new ligand binding and release mechanism., Lartigue A, Gruez A, Spinelli S, Riviere S, Brossut R, Tegoni M, Cambillau C, J Biol Chem. 2003 Aug 8;278(32):30213-8. Epub 2003 May 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12766173 12766173]
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</StructureSection>
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[[Category: Leucophaea maderae]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Rhyparobia maderae]]
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[[Category: Brossut, R.]]
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[[Category: Brossut R]]
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[[Category: Cambillau, C.]]
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[[Category: Cambillau C]]
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[[Category: Gruez, A.]]
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[[Category: Gruez A]]
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[[Category: Lartigue, A.]]
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[[Category: Lartigue A]]
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[[Category: Riviere, S.]]
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[[Category: Riviere S]]
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[[Category: Spinelli, S.]]
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[[Category: Spinelli S]]
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[[Category: Tegoni, M.]]
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[[Category: Tegoni M]]
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[[Category: ligand 8-anilino-1-naphtalenesulfonic acid]]
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[[Category: pheromone binding protein]]
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[[Category: transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:51:13 2008''
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Current revision

Crystal structure of a pheromone binding protein from the cockroach Leucophaea maderae in complex with the fluorescent reporter ANS (1-anilinonaphtalene-8-sulfonic acid),

PDB ID 1ow4

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