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1p5d

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(New page: 200px<br /><applet load="1p5d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p5d, resolution 1.60&Aring;" /> '''Enzyme-ligand comple...)
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[[Image:1p5d.jpg|left|200px]]<br /><applet load="1p5d" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p5d, resolution 1.60&Aring;" />
 
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'''Enzyme-ligand complex of P. aeruginosa PMM/PGM'''<br />
 
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==Overview==
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==Enzyme-ligand complex of P. aeruginosa PMM/PGM==
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Enzyme-substrate complexes of phosphomannomutase/phosphoglucomutase, (PMM/PGM) reveal the structural basis of the enzyme's ability to use four, different substrates in catalysis. High-resolution structures with glucose, 1-phosphate, glucose 6-phosphate, mannose 1-phosphate, and mannose, 6-phosphate show that the position of the phosphate group of each, substrate is held constant by a conserved network of hydrogen bonds. This, produces two distinct, and mutually exclusive, binding orientations for, the sugar rings of the 1-phospho and 6-phospho sugars. Specific binding of, both orientations is accomplished by key contacts with the O3 and O4, hydroxyls of the sugar, which must occupy equatorial positions. Dual, recognition of glucose and mannose phosphosugars uses a combination of, specific protein contacts and nonspecific solvent contacts. The ability of, PMM/PGM to accommodate these four diverse substrates in a single active, site is consistent with its highly reversible phosphoryl transfer reaction, and allows it to function in multiple biosynthetic pathways in P., aeruginosa.
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<StructureSection load='1p5d' size='340' side='right'caption='[[1p5d]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P5D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p5d OCA], [https://pdbe.org/1p5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p5d RCSB], [https://www.ebi.ac.uk/pdbsum/1p5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p5d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALGC_PSEAE ALGC_PSEAE] The phosphomannomutase activity produces a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics. Also involved in core-LPS biosynthesis due to its phosphoglucomutase activity. Essential for rhamnolipid production, an exoproduct correlated with pathogenicity, and for biofilm production.<ref>PMID:7515870</ref> <ref>PMID:10481091</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p5/1p5d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p5d ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1P5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with G1P and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphomannomutase Phosphomannomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.8 5.4.2.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P5D OCA].
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*[[Phosphomannomutase|Phosphomannomutase]]
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== References ==
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==Reference==
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<references/>
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Structural basis of diverse substrate recognition by the enzyme PMM/PGM from P. aeruginosa., Regni C, Naught L, Tipton PA, Beamer LJ, Structure. 2004 Jan;12(1):55-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14725765 14725765]
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__TOC__
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[[Category: Phosphomannomutase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Beamer LJ]]
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[[Category: Beamer, L.J.]]
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[[Category: Regni C]]
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[[Category: Regni, C.]]
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[[Category: Tipton PA]]
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[[Category: Tipton, P.A.]]
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[[Category: G1P]]
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[[Category: ZN]]
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[[Category: alpha/beta protein]]
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[[Category: enzyme-ligand complex]]
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[[Category: enzyme-metal complex]]
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[[Category: phosphohexomutase]]
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[[Category: phosphoserine]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:29:15 2007''
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Current revision

Enzyme-ligand complex of P. aeruginosa PMM/PGM

PDB ID 1p5d

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