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1pbi

From Proteopedia

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(New page: 200px<br /><applet load="1pbi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pbi, resolution 2.70&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1pbi.jpg|left|200px]]<br /><applet load="1pbi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pbi, resolution 2.70&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A BOWMAN-BIRK INHIBITOR FROM PEA SEEDS'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A BOWMAN-BIRK INHIBITOR FROM PEA SEEDS==
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The trypsin/chymotrypsin inhibitors from winter pea seeds (PsTI) are, members of the Bowman-Birk protease inhibitor (BBPI) family. The crystal, structure of the isoform PsTI-IVb was determined by molecular replacement, at 2.7 A resolution using the X-ray co-ordinates of the soybean inhibitor, as a search model. The inhibitor crystallized with a nearly perfect 2-fold, symmetric dimer in the asymmetric unit. Although the overall structure is, very similar to that seen in other BBPIs, there are notable new structural, features. Unlike the previously reported X-ray structures of BBPIs, the, structure of PsTI-IVb includes the C-terminal segment of the molecule. The, C-terminal tail of each subunit is partly beta-stranded and interacts with, the 2-fold symmetry-related subunit, forming a beta-sheet with strands A, and B of this subunit. The dimer is mainly stabilized by a large internal, hydrogen-bonded network surrounded by two hydrophobic links. Fluorescence, anisotropy decay measurements show that residues Tyr59 and Tyr43 are, mobile in the picosecond time scale with a large amplitude. The, fluorescence study and a molecular model of the simultaneous binding of, PsTI-IVb to porcine trypsin and bovine chymotrypsin are compatible only, with a monomeric state of the functional molecule in solution.
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<StructureSection load='1pbi' size='340' side='right'caption='[[1pbi]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1pbi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PBI FirstGlance]. <br>
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1PBI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PBI OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pbi OCA], [https://pdbe.org/1pbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pbi RCSB], [https://www.ebi.ac.uk/pdbsum/1pbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pbi ProSAT]</span></td></tr>
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==Reference==
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</table>
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Dimeric crystal structure of a Bowman-Birk protease inhibitor from pea seeds., Li de la Sierra I, Quillien L, Flecker P, Gueguen J, Brunie S, J Mol Biol. 1999 Jan 22;285(3):1195-207. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9887273 9887273]
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== Function ==
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[https://www.uniprot.org/uniprot/IBBB_PEA IBBB_PEA] Inhibitor of trypsin and of chymotrypsin. May function as a natural phytochemical defense against predators.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pb/1pbi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pbi ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pisum sativum]]
[[Category: Pisum sativum]]
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[[Category: Single protein]]
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[[Category: Brunie S]]
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[[Category: Brunie, S.]]
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[[Category: Li De La Sierra I]]
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[[Category: Sierra, I.Li.De.La.]]
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[[Category: bowman-birk inhibitor]]
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[[Category: chymotrypsin inhibitor]]
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[[Category: trypsin inhibitor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:39:58 2007''
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Current revision

CRYSTAL STRUCTURE OF A BOWMAN-BIRK INHIBITOR FROM PEA SEEDS

PDB ID 1pbi

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