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1s4e

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(New page: 200px<br /><applet load="1s4e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s4e, resolution 2.90&Aring;" /> '''Pyrococcus furiosus ...)
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[[Image:1s4e.gif|left|200px]]<br /><applet load="1s4e" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s4e, resolution 2.90&Aring;" />
 
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'''Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium'''<br />
 
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==Overview==
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==Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium==
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Galactokinase (GalK) catalyses the first step of the Leloir pathway of, galactose metabolism, the ATP-dependent phosphorylation of galactose to, galactose-1-phosphate. In man, defects in galactose metabolism can result, in disorders with severe clinical consequences, and deficiencies in, galactokinase have been linked with the development of cataracts within, the first few months of life. The crystal structure of GalK from, Pyrococcus furiosus in complex with MgADP and galactose has been, determined to 2.9 A resolution to provide insights into the substrate, specificity and catalytic mechanism of the enzyme. The structure consists, of two domains with the active site in a cleft at the domain interface., Inspection of the substrate binding pocket identifies the amino acid, residues involved in galactose and nucleotide binding and points to both, structural and mechanistic similarities with other enzymes of the GHMP, kinase superfamily to which GalK belongs. Comparison of the sequence of, the Gal3p inducer protein, which is related to GalK and which forms part, of the transcriptional activation of the GAL gene cluster in the yeast, Saccharomyces cerevisiae, has led to an understanding of the molecular, basis of galactose and nucleotide recognition. Finally, the structure has, enabled us to further our understanding on the functional consequences of, mutations in human GalK which cause galactosemia.
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<StructureSection load='1s4e' size='340' side='right'caption='[[1s4e]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1s4e]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S4E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4e OCA], [https://pdbe.org/1s4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s4e RCSB], [https://www.ebi.ac.uk/pdbsum/1s4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s4e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAL1_PYRFU GAL1_PYRFU] Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). Is very specific for its substrate, since it is not able to use D-glucose, D-fructose, D-mannose, 2-deoxy-D-glucose, and D-glucosamine as substrates.<ref>PMID:11978175</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s4/1s4e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s4e ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1S4E is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with GLA, MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Galactokinase Galactokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.6 2.7.1.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S4E OCA].
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*[[Galactokinase|Galactokinase]]
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== References ==
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==Reference==
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<references/>
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Substrate specificity and mechanism from the structure of Pyrococcus furiosus galactokinase., Hartley A, Glynn SE, Barynin V, Baker PJ, Sedelnikova SE, Verhees C, de Geus D, van der Oost J, Timson DJ, Reece RJ, Rice DW, J Mol Biol. 2004 Mar 19;337(2):387-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15003454 15003454]
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__TOC__
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[[Category: Galactokinase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: Single protein]]
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[[Category: Baker PJ]]
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[[Category: Baker, P.J.]]
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[[Category: Barynin V]]
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[[Category: Barynin, V.]]
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[[Category: Glynn SE]]
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[[Category: Geus, D.de.]]
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[[Category: Hartley A]]
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[[Category: Glynn, S.E.]]
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[[Category: Reece RJ]]
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[[Category: Hartley, A.]]
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[[Category: Rice DW]]
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[[Category: Oost, J.van.der.]]
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[[Category: Sedelnikova SE]]
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[[Category: Reece, R.J.]]
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[[Category: Timson DJ]]
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[[Category: Rice, D.W.]]
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[[Category: Verhees C]]
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[[Category: Sedelnikova, S.E.]]
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[[Category: De Geus D]]
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[[Category: Timson, D.J.]]
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[[Category: Van der Oost J]]
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[[Category: Verhees, C.]]
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[[Category: ADP]]
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[[Category: GLA]]
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[[Category: MG]]
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[[Category: ghmp kinase superfamily]]
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[[Category: p-loop]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:05:31 2007''
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Current revision

Pyrococcus furiosus galactokinase in complex with galactose, ADP and magnesium

PDB ID 1s4e

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