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1wbl

From Proteopedia

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[[Image:1wbl.jpg|left|200px]]
 
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{{Structure
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==WINGED BEAN LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE==
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|PDB= 1wbl |SIZE=350|CAPTION= <scene name='initialview01'>1wbl</scene>, resolution 2.5&Aring;
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<StructureSection load='1wbl' size='340' side='right'caption='[[1wbl]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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<table><tr><td colspan='2'>[[1wbl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WBL FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbl OCA], [https://pdbe.org/1wbl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wbl RCSB], [https://www.ebi.ac.uk/pdbsum/1wbl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wbl ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbl OCA], [http://www.ebi.ac.uk/pdbsum/1wbl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wbl RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/LEC1_PSOTE LEC1_PSOTE] Lectin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wb/1wbl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wbl ConSurf].
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<div style="clear:both"></div>
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'''WINGED BEAN LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE'''
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==See Also==
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The structure of basic Winged Bean Agglutinin (WBAI) with two dimeric molecules complexed with methyl-alpha-D-galactopyranoside in the asymmetric unit, has been determined by the molecular replacement method and refined with 2.5 A X-ray intensity data. The polypeptide chain of each monomer has the characteristic legume lectin tertiary fold. The structure clearly defines the lectin-carbohydrate interactions. It reveals how the unusually long variable loop in the binding region endows the lectin with its characteristic sugar specificity. The lectin forms non-canonical dimers of the type found in Erythrina corallodendron lectin (EcorL) even though glycosylation, unlike in EcorL, does not prevent the formation of canonical dimers. The structure thus further demonstrates that the mode of dimerisation of legume lectins is not necessarily determined by the covalently bound carbohydrate but is governed by features intrinsic to the protein. The present analysis and our earlier work on peanut lectin (PNA), show that legume lectins are a family of proteins in which small alterations in essentially the same tertiary structure lead to wide variations in quaternary association. A relationship among the non-canonical modes of dimeric association in legume lectins is presented.
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[[Category: Large Structures]]
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==About this Structure==
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1WBL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBL OCA].
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==Reference==
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Carbohydrate specificity and quaternary association in basic winged bean lectin: X-ray analysis of the lectin at 2.5 A resolution., Prabu MM, Sankaranarayanan R, Puri KD, Sharma V, Surolia A, Vijayan M, Suguna K, J Mol Biol. 1998 Mar 6;276(4):787-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9500920 9500920]
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[[Category: Psophocarpus tetragonolobus]]
[[Category: Psophocarpus tetragonolobus]]
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[[Category: Single protein]]
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[[Category: Prabu MM]]
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[[Category: Prabu, M M.]]
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[[Category: Puri KD]]
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[[Category: Puri, K D.]]
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[[Category: Sankaranarayanan R]]
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[[Category: Sankaranarayanan, R.]]
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[[Category: Sharma V]]
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[[Category: Sharma, V.]]
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[[Category: Suguna K]]
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[[Category: Suguna, K.]]
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[[Category: Surolia A]]
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[[Category: Surolia, A.]]
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[[Category: Vijayan M]]
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[[Category: Vijayan, M.]]
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[[Category: agglutinin]]
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[[Category: blood group specificity]]
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[[Category: glycosylated protein]]
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[[Category: legume lectin]]
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[[Category: quaternary association]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:34:01 2008''
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Current revision

WINGED BEAN LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE

PDB ID 1wbl

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