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1xds

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{{Seed}}
 
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[[Image:1xds.png|left|200px]]
 
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==Crystal structure of Aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM) and 11-deoxy-beta-rhodomycin (DbrA)==
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The line below this paragraph, containing "STRUCTURE_1xds", creates the "Structure Box" on the page.
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<StructureSection load='1xds' size='340' side='right'caption='[[1xds]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xds]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XDS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DRA:11-DEOXY-BETA-RHODOMYCIN'>DRA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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{{STRUCTURE_1xds| PDB=1xds | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xds OCA], [https://pdbe.org/1xds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xds RCSB], [https://www.ebi.ac.uk/pdbsum/1xds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xds ProSAT]</span></td></tr>
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</table>
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===Crystal structure of Aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM) and 11-deoxy-beta-rhodomycin (DbrA)===
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== Function ==
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[https://www.uniprot.org/uniprot/RDMB_STREF RDMB_STREF] Involved in the biosynthesis of the anthracycline aclacinomycin which is an aromatic polyketide antibiotic that exhibits high cytotoxicity and is widely applied in the chemotherapy of a variety of cancers. In vivo and in vitro, RdmB catalyzes the removal of the carboxylic group from the C-10 position of 15-demethoxyaclacinomycin T coupled to hydroxylation at the same C-10 position. It could also catalyze the removal of the carboxylic group at the C-10 position of 15-demethoxy-epsilon-rhodomycin coupled to hydroxylation at the same C-10 position to yield rhodomycin B. The reaction catalyzes by RdmB is intriguing, since the enzyme does not use any of the cofactors usually associated with hydroxylases such as flavins and/or metal ions to activate molecular oxygen.<ref>PMID:11004563</ref> <ref>PMID:15548527</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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The line below this paragraph, {{ABSTRACT_PUBMED_15548527}}, adds the Publication Abstract to the page
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Check<jmol>
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(as it appears on PubMed at http://www.pubmed.gov), where 15548527 is the PubMed ID number.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xd/1xds_consurf.spt"</scriptWhenChecked>
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{{ABSTRACT_PUBMED_15548527}}
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1XDS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XDS OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xds ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Aclacinomycin 10-hydroxylase is a novel substrate-assisted hydroxylase requiring S-adenosyl-L-methionine as cofactor., Jansson A, Koskiniemi H, Erola A, Wang J, Mantsala P, Schneider G, Niemi J, J Biol Chem. 2005 Feb 4;280(5):3636-44. Epub 2004 Nov 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15548527 15548527]
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<references/>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces purpurascens]]
[[Category: Streptomyces purpurascens]]
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[[Category: Erola, A.]]
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[[Category: Erola A]]
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[[Category: Jansson, A.]]
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[[Category: Jansson A]]
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[[Category: Koskiniemi, H.]]
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[[Category: Koskiniemi H]]
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[[Category: Mantsala, P.]]
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[[Category: Mantsala P]]
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[[Category: Niemi, J.]]
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[[Category: Niemi J]]
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[[Category: SPINE, Structural Proteomics in Europe.]]
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[[Category: Schneider G]]
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[[Category: Schneider, G.]]
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[[Category: Wang J]]
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[[Category: Wang, J.]]
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[[Category: Anthracycline]]
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[[Category: Divergent evolution]]
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[[Category: Hydroxylase]]
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[[Category: Polyketide antibiotic]]
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[[Category: S-adenosyl-l-methionine]]
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[[Category: Spine]]
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[[Category: Streptomyce]]
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[[Category: Structural genomic]]
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[[Category: Structural proteomics in europe]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:19:56 2008''
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Current revision

Crystal structure of Aclacinomycin-10-hydroxylase (RdmB) in complex with S-adenosyl-L-methionine (SAM) and 11-deoxy-beta-rhodomycin (DbrA)

PDB ID 1xds

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