1xvq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:19, 3 April 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1xvq.gif|left|200px]]
 
-
<!--
+
==Crystal structure of thiol peroxidase from Mycobacterium tuberculosis==
-
The line below this paragraph, containing "STRUCTURE_1xvq", creates the "Structure Box" on the page.
+
<StructureSection load='1xvq' size='340' side='right'caption='[[1xvq]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1xvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XVQ FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
-
{{STRUCTURE_1xvq| PDB=1xvq | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xvq OCA], [https://pdbe.org/1xvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xvq RCSB], [https://www.ebi.ac.uk/pdbsum/1xvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xvq ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/TPX_MYCTU TPX_MYCTU] Has antioxidant activity. Could remove peroxides or H(2)O(2) (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xv/1xvq_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xvq ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal structure of thiol peroxidase from Mycobacterium tuberculosis'''
+
==See Also==
-
 
+
*[[Thiol peroxidase 3D structures|Thiol peroxidase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
A thiol peroxidase (Tpx) from Mycobacterium tuberculosis was functionally analyzed. The enzyme shows NADPH-linked peroxidase activity using a thioredoxin-thioredoxin reductase system as electron donor, and anti-oxidant activity in a thiol-dependent metal-catalyzed oxidation system. It reduces H2O2, t-butyl hydroperoxide, and cumene hydroperoxide, and is inhibited by sulfhydryl reagents. Mutational studies revealed that the peroxidatic (Cys60) and resolving (Cys93) cysteine residues are critical amino acids for catalytic activity. The X-ray structure determined to a resolution of 1.75 A shows a thioredoxin fold similar to that of other peroxiredoxin family members. Superposition with structural homologues in oxidized and reduced forms indicates that the M. tuberculosis Tpx is a member of the atypical two-Cys peroxiredoxin family. In addition, the short distance that separates the Calpha atoms of Cys60 and Cys93 and the location of these cysteine residues in unstructured regions may indicate that the M. tuberculosis enzyme is oxidized, though the side-chain of Cys60 is poorly visible. It is solely in the reduced Streptococcus pneumoniae Tpx structure that both residues are part of two distinct helical segments. The M. tuberculosis Tpx is dimeric both in solution and in the crystal structure. Amino acid residues from both monomers delineate the active site pocket.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1XVQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XVQ OCA].
+
-
 
+
-
==Reference==
+
-
Functional and structural characterization of a thiol peroxidase from Mycobacterium tuberculosis., Rho BS, Hung LW, Holton JM, Vigil D, Kim SI, Park MS, Terwilliger TC, Pedelacq JD, J Mol Biol. 2006 Sep 1;361(5):850-63. Epub 2006 Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16884737 16884737]
+
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
-
[[Category: Single protein]]
+
[[Category: Holton JM]]
-
[[Category: Holton, J M.]]
+
[[Category: Hung LW]]
-
[[Category: Hung, L W.]]
+
[[Category: Kim SI]]
-
[[Category: Kim, S I.]]
+
[[Category: Park MS]]
-
[[Category: Park, M S.]]
+
[[Category: Pedelacq JD]]
-
[[Category: Pedelacq, J D.]]
+
[[Category: Rho BS]]
-
[[Category: Rho, B S.]]
+
[[Category: Terwilliger TC]]
-
[[Category: TBSGC, TB Structural Genomics Consortium.]]
+
[[Category: Vigil D]]
-
[[Category: Terwilliger, T C.]]
+
-
[[Category: Vigil, D.]]
+
-
[[Category: Protein structure initiative]]
+
-
[[Category: Psi]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: Tb structural genomics consortium]]
+
-
[[Category: Tbsgc]]
+
-
[[Category: Thioredoxin fold]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:34:04 2008''
+

Current revision

Crystal structure of thiol peroxidase from Mycobacterium tuberculosis

PDB ID 1xvq

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xvq"
Personal tools