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2a8x

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[[Image:2a8x.png|left|200px]]
 
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{{STRUCTURE_2a8x| PDB=2a8x | SCENE= }}
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==Crystal Structure of Lipoamide Dehydrogenase from Mycobacterium tuberculosis==
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<StructureSection load='2a8x' size='340' side='right'caption='[[2a8x]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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===Crystal Structure of Lipoamide Dehydrogenase from Mycobacterium tuberculosis===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2a8x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A8X FirstGlance]. <br>
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{{ABSTRACT_PUBMED_16093239}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a8x OCA], [https://pdbe.org/2a8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a8x RCSB], [https://www.ebi.ac.uk/pdbsum/2a8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a8x ProSAT]</span></td></tr>
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[[2a8x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A8X OCA].
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DLDH_MYCTU DLDH_MYCTU] Lipoamide dehydrogenase is an essential component of the alpha-ketoacid dehydrogenase complexes, namely the pyruvate dehydrogenase (PDH) complex, the branched-chain alpha-ketoacid dehydrogenase (BCKADH) complex, and likely also the 2-oxoglutarate dehydrogenase (ODH) complex. Catalyzes the reoxidation of dihydrolipoyl groups which are covalently attached to the lipoate acyltransferase components (E2) of the complexes. Is also able to catalyze the transhydrogenation of NADH and thio-NAD(+) in the absence of D,L-lipoamide, and the NADH-dependent reduction of quinones in vitro.<ref>PMID:11560483</ref> <ref>PMID:11799204</ref> <ref>PMID:16045627</ref> <ref>PMID:21238944</ref> <ref>PMID:16093239</ref> Together with AhpC, AhpD and DlaT, Lpd constitutes an NADH-dependent peroxidase active against hydrogen and alkyl peroxides as well as serving as a peroxynitrite reductase, thus protecting the bacterium against reactive nitrogen intermediates and oxidative stress generated by the host immune system.<ref>PMID:11560483</ref> <ref>PMID:11799204</ref> <ref>PMID:16045627</ref> <ref>PMID:21238944</ref> <ref>PMID:16093239</ref> Appears to be essential for Mtb pathogenesis.<ref>PMID:11560483</ref> <ref>PMID:11799204</ref> <ref>PMID:16045627</ref> <ref>PMID:21238944</ref> <ref>PMID:16093239</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a8/2a8x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a8x ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
*[[Dihydrolipoamide dehydrogenase|Dihydrolipoamide dehydrogenase]]
*[[Dihydrolipoamide dehydrogenase|Dihydrolipoamide dehydrogenase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016093239</ref><references group="xtra"/>
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__TOC__
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[[Category: Dihydrolipoyl dehydrogenase]]
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</StructureSection>
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Large Structures]]
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[[Category: Bryk, R.]]
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[[Category: Buglino, J A.]]
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[[Category: Kniewel, R.]]
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[[Category: Lima, C D.]]
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[[Category: Nathan, C F.]]
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[[Category: Rajashankar, K R.]]
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[[Category: Alpha keto acid dehydrogenase]]
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[[Category: Lipoamide dehydrogenase]]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Oxidoreductase]]
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[[Category: Bryk R]]
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[[Category: Pyruvate dehydrogenase]]
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[[Category: Buglino JA]]
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[[Category: Kniewel R]]
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[[Category: Lima CD]]
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[[Category: Nathan CF]]
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[[Category: Rajashankar KR]]

Current revision

Crystal Structure of Lipoamide Dehydrogenase from Mycobacterium tuberculosis

PDB ID 2a8x

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