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2bb6

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(New page: 200px<br /><applet load="2bb6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bb6, resolution 2.00&Aring;" /> '''Structure of Cobalam...)
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[[Image:2bb6.gif|left|200px]]<br /><applet load="2bb6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bb6, resolution 2.00&Aring;" />
 
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'''Structure of Cobalamin-complexed Bovine Transcobalamin in Monoclinic Crystal Form'''<br />
 
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==Overview==
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==Structure of Cobalamin-complexed Bovine Transcobalamin in Monoclinic Crystal Form==
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Cobalamin (Cbl, vitamin B(12)) serves for two essential cofactors in, mammals. The pathway for its intestinal absorption, plasma transport, and, cellular uptake uses cell surface receptors and three Cbl-transporting, proteins, haptocorrin, intrinsic factor, and transcobalamin (TC). We, present the structure determination of a member of the mammalian, Cbl-transporter family. The crystal structures of recombinant human and, bovine holo-TCs reveal a two-domain architecture, with an N-terminal, alpha(6)-alpha(6) barrel and a smaller C-terminal domain. One Cbl molecule, in base-on conformation is buried inside the domain interface. Structural, data combined with previous binding assays indicate a domain motion in the, first step of Cbl binding. In a second step, the weakly coordinated ligand, H(2)O at the upper axial side of added H(2)O-Cbl is displaced by a, histidine residue of the alpha(6)-alpha(6) barrel. Analysis of amino acid, conservation on TC's surface in orthologous proteins suggests the location, of the TC-receptor-recognition site in an extended region on the, alpha(6)-alpha(6) barrel. The TC structure allows for the mapping of sites, of amino acid variation due to polymorphisms of the human TC gene., Structural information is used to predict the overall fold of haptocorrin, and intrinsic factor and permits a rational approach to the design of new, Cbl-based bioconjugates for diagnostic or therapeutic drug delivery.
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<StructureSection load='2bb6' size='340' side='right'caption='[[2bb6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2bb6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BB6 FirstGlance]. <br>
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2BB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CL and B12 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BB6 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb6 OCA], [https://pdbe.org/2bb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bb6 RCSB], [https://www.ebi.ac.uk/pdbsum/2bb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bb6 ProSAT]</span></td></tr>
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Structural basis for mammalian vitamin B12 transport by transcobalamin., Wuerges J, Garau G, Geremia S, Fedosov SN, Petersen TE, Randaccio L, Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4386-91. Epub 2006 Mar 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16537422 16537422]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TCO2_BOVIN TCO2_BOVIN] Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/2bb6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bb6 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fedosov, S.N.]]
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[[Category: Fedosov SN]]
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[[Category: Garau, G.]]
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[[Category: Garau G]]
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[[Category: Geremia, S.]]
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[[Category: Geremia S]]
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[[Category: Petersen, T.E.]]
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[[Category: Petersen TE]]
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[[Category: Randaccio, L.]]
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[[Category: Randaccio L]]
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[[Category: Wuerges, J.]]
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[[Category: Wuerges J]]
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[[Category: B12]]
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[[Category: CL]]
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[[Category: alpha_6 - alpha_6 barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:41:30 2007''
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Current revision

Structure of Cobalamin-complexed Bovine Transcobalamin in Monoclinic Crystal Form

PDB ID 2bb6

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