This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2era

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

RECOMBINANT ERABUTOXIN A, S8G MUTANT

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2era"

@@ Line 1: / Line 1: @@
-[[Image:2era.jpg|left|200px]]
- {{Structure
+==RECOMBINANT ERABUTOXIN A, S8G MUTANT==
-|PDB= 2era |SIZE=350|CAPTION= <scene name='initialview01'>2era</scene>, resolution 1.81&Aring;
+<StructureSection load='2era' size='340' side='right'caption='[[2era]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[2era]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ERA FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
-|GENE=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2era OCA], [https://pdbe.org/2era PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2era RCSB], [https://www.ebi.ac.uk/pdbsum/2era PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2era ProSAT]</span></td></tr>
-|DOMAIN=
+</table>
-|RELATEDENTRY=
+== Function ==
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2era FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2era OCA], [http://www.ebi.ac.uk/pdbsum/2era PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2era RCSB]</span>
+[https://www.uniprot.org/uniprot/3S1EA_LATSE 3S1EA_LATSE] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) (tested on Torpedo marmorata, Kd=21 uM) and with low affinity to neuronal alpha-7/CHRNA7 nAChRs (tested on chimeric alpha-7/CHRNA7, Kd=0.07 nM) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission (PubMed:7721859). Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations (PubMed:9840221). In vivo, produces peripheral paralysis.<ref>PMID:7721859</ref> <ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
-}}
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
-'''RECOMBINANT ERABUTOXIN A, S8G MUTANT'''
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/2era_consurf.spt"</scriptWhenChecked>
-==Overview==
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-A previous mutational analysis of erabutoxin a (Ea), a curaremimetic toxin from sea snake venom, showed that the substitutions S8G and S8T caused, respectively, 176-fold and 780-fold affinity decreases for the nicotinic acetylcholine receptor (AchR). In view of the fact that the side-chain of Ser8 is buried in the wild-type toxin, we wondered whether these affinity changes reflect a direct binding contribution of S8 to the receptor and/or conformational changes that could have occurred in Ea as a result of the introduced mutations. To approach this question, we solved X-ray structures of the two mutants S8G and S8T at high resolution (0.18 nm and 0.17 nm, with R factors of 18.0% and 17.9%, respectively). The data show that none of the mutations significantly modified the toxin structure. Even within the site where the toxin binds to the receptor the backbone conformation remained unchanged. Therefore, the low affinities of the mutants S8T and S8G cannot be explained by a large conformational change of the toxin structure. Although we cannot exclude the possibility that undetectable structural changes have occurred in the toxin mutants, our data support the view that, although buried between loop I and II, S8 is part of the functional epitope of the toxin.
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
-==About this Structure==
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2era ConSurf].
-ERA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Laticauda_semifasciata Laticauda semifasciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERA OCA].
+<div style="clear:both"></div>
+== References ==
-==Reference==
+<references/>
-High resolution x-ray analysis of two mutants of a curaremimetic snake toxin., Gaucher JF, Menez R, Arnoux B, Pusset J, Ducruix A, Eur J Biochem. 2000 Mar;267(5):1323-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10691969 10691969]
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Laticauda semifasciata]]
-[[Category: Single protein]]
+[[Category: Arnoux B]]
-[[Category: Arnoux, B.]]
+[[Category: Ducruix A]]
-[[Category: Ducruix, A.]]
+[[Category: Gaucher JF]]
-[[Category: Gaucher, J F.]]
+[[Category: Menez A]]
-[[Category: Menez, A.]]
+[[Category: Menez R]]
-[[Category: Menez, R.]]
-[[Category: postsynaptic neurotoxin]]
-[[Category: snake neurotoxin]]
-[[Category: venom]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:52:43 2008''

2era

From Proteopedia

Current revision

RECOMBINANT ERABUTOXIN A, S8G MUTANT

Structural highlights

Function

Evolutionary Conservation

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox