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2ntt

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[[Image:2ntt.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of SEK==
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|PDB= 2ntt |SIZE=350|CAPTION= <scene name='initialview01'>2ntt</scene>, resolution 1.561&Aring;
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<StructureSection load='2ntt' size='340' side='right'caption='[[2ntt]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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<table><tr><td colspan='2'>[[2ntt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTT FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.561&#8491;</td></tr>
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|GENE= sek ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntt OCA], [https://pdbe.org/2ntt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntt RCSB], [https://www.ebi.ac.uk/pdbsum/2ntt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntt ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of SEK'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q93CC5_STAAU Q93CC5_STAAU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Superantigens (SAGs) interact with host immune receptors to induce a massive release of inflammatory cytokines that can lead to toxic shock syndrome and death. Bacterial SAGs can be classified into five distinct evolutionary groups. Group V SAGs are characterized by the alpha3-beta8 loop, a unique approximately 15 amino acid residue extension that is required for optimal T cell activation. Here, we report the X-ray crystal structures of the group V SAG staphylococcal enterotoxin K (SEK) alone and in complex with the TCR hVbeta5.1 domain. SEK adopts a unique TCR binding orientation relative to other SAG-TCR complexes, which results in the alpha3-beta8 loop contacting the apical loop of framework region 4, thereby extending the known TCR recognition site of SAGs. These interactions are absolutely required for TCR binding and T cell activation by SEK, and dictate the TCR Vbeta domain specificity of SEK and other group V SAGs.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2ntt_consurf.spt"</scriptWhenChecked>
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2NTT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTT OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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A novel loop domain in superantigens extends their T cell receptor recognition site., Gunther S, Varma AK, Moza B, Kasper KJ, Wyatt AW, Zhu P, Rahman AK, Li Y, Mariuzza RA, McCormick JK, Sundberg EJ, J Mol Biol. 2007 Aug 3;371(1):210-21. Epub 2007 May 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17560605 17560605]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ntt ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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[[Category: Staphylococcus aureus]]
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__TOC__
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[[Category: Gunther, S.]]
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</StructureSection>
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[[Category: Moza, B.]]
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[[Category: Large Structures]]
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[[Category: Sundberg, E J.]]
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[[Category: Staphylococcus aureus subsp. aureus COL]]
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[[Category: Varma, A K.]]
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[[Category: Gunther S]]
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[[Category: CL]]
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[[Category: Moza B]]
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[[Category: EDO]]
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[[Category: Sundberg EJ]]
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[[Category: superantigen; t cell receptor]]
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[[Category: Varma AK]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:51:04 2008''
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Current revision

Crystal Structure of SEK

PDB ID 2ntt

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