This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2ovw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:30, 3 April 2024) (edit) (undo)
 
(21 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ovw.gif|left|200px]]<br />
 
-
<applet load="2ovw" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2ovw, resolution 2.3&Aring;" />
 
-
'''ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE'''<br />
 
-
==Overview==
+
==ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE==
-
The mechanisms involved in the enzymatic degradation of cellulose are of, great ecological and commercial importance. The breakdown of cellulose by, fungal species is performed by a consortium of free enzymes, known as, cellobiohydrolases and endoglucanases, which are found in many of the 57, glycosyl hydrolase families. The structure of the endoglucanase I (EG I), found in glycosyl hydrolase family 7, from the thermophilic fungus, Fusarium oxysporum has been solved at 2.3 A resolution. In addition to the, native enzyme, structures have also been determined with both the affinity, label, 3,4-epoxybutyl beta-D-cellobioside, and the reaction product, cellobiose. The affinity label is covalently bound, as expected, to the, catalytic nucleophile, Glu197, with clear evidence for binding of ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9153432 (full description)]]
+
<StructureSection load='2ovw' size='340' side='right'caption='[[2ovw]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2ovw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OVW FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PRD_900005:beta-cellobiose'>PRD_900005</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ovw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ovw OCA], [https://pdbe.org/2ovw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ovw RCSB], [https://www.ebi.ac.uk/pdbsum/2ovw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ovw ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GUNC_FUSOX GUNC_FUSOX]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ov/2ovw_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ovw ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
2OVW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]] with NAG and CBI as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Sites: CTA, CTB, CTC and CTD. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2OVW OCA]].
+
*[[Glucanase 3D structures|Glucanase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure of the endoglucanase I from Fusarium oxysporum: native, cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3 A resolution., Sulzenbacher G, Schulein M, Davies GJ, Biochemistry. 1997 May 13;36(19):5902-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9153432 9153432]
+
-
[[Category: Cellulase]]
+
[[Category: Fusarium oxysporum]]
[[Category: Fusarium oxysporum]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Davies, G.J.]]
+
[[Category: Davies GJ]]
-
[[Category: Schulein, M.]]
+
[[Category: Schulein M]]
-
[[Category: Sulzenbacher, G.]]
+
[[Category: Sulzenbacher G]]
-
[[Category: CBI]]
+
-
[[Category: NAG]]
+
-
[[Category: complexed with cellobiose]]
+
-
[[Category: endoglucanase i]]
+
-
[[Category: glycosyl hydrolase]]
+
-
[[Category: glycosylated protein]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:06:03 2007''
+

Current revision

ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE

PDB ID 2ovw

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ovw"
Personal tools