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2p0b

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[[Image:2p0b.jpg|left|200px]]
 
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==Crystal structure of chemically-reduced E.coli nrfB==
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The line below this paragraph, containing "STRUCTURE_2p0b", creates the "Structure Box" on the page.
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<StructureSection load='2p0b' size='340' side='right'caption='[[2p0b]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2p0b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P0B FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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{{STRUCTURE_2p0b| PDB=2p0b | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p0b OCA], [https://pdbe.org/2p0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p0b RCSB], [https://www.ebi.ac.uk/pdbsum/2p0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p0b ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of chemically-reduced E.coli nrfB'''
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== Function ==
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[https://www.uniprot.org/uniprot/NRFB_ECOLI NRFB_ECOLI] Plays a role in nitrite reduction.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 A (1 A=0.1 nm) resolution. The architecture of the protein is that of a 40 A 'nanowire' in which the five haems are positioned within 6 A of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p0/2p0b_consurf.spt"</scriptWhenChecked>
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2P0B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P0B OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA., Clarke TA, Cole JA, Richardson DJ, Hemmings AM, Biochem J. 2007 Aug 15;406(1):19-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17521287 17521287]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p0b ConSurf].
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[[Category: Escherichia coli]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Clarke, T A.]]
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</StructureSection>
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[[Category: Hemmings, A M.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Richardson, D J.]]
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[[Category: Large Structures]]
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[[Category: Electron transport]]
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[[Category: Clarke TA]]
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[[Category: Pentaheme c-type cytochrome]]
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[[Category: Hemmings AM]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:03:09 2008''
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[[Category: Richardson DJ]]

Current revision

Crystal structure of chemically-reduced E.coli nrfB

PDB ID 2p0b

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