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2z8g

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[[Image:2z8g.jpg|left|200px]]
 
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{{Structure
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==Aspergillus niger ATCC9642 isopullulanase complexed with isopanose==
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|PDB= 2z8g |SIZE=350|CAPTION= <scene name='initialview01'>2z8g</scene>, resolution 1.70&Aring;
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<StructureSection load='2z8g' size='340' side='right'caption='[[2z8g]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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<table><tr><td colspan='2'>[[2z8g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z8G FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Isopullulanase Isopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.57 3.2.1.57]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8g OCA], [https://pdbe.org/2z8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z8g RCSB], [https://www.ebi.ac.uk/pdbsum/2z8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z8g ProSAT]</span></td></tr>
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</table>
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'''Aspergillus niger ATCC9642 isopullulanase complexed with isopanose'''
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== Function ==
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[https://www.uniprot.org/uniprot/IPUA_ASPNG IPUA_ASPNG] Hydrolyzes pullulan, a linear polymer which is composed of maltotriose units with alpha-1,6 glucosidic linkages, to produce isopanose (Glca1-4Glca1-6Glc).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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An isopullulanase (IPU) from Aspergillus niger ATCC9642 hydrolyzes alpha-1,4-glucosidic linkages of pullulan to produce isopanose. Although IPU does not hydrolyze dextran, it is classified into glycoside hydrolase family 49 (GH49), major members of which are dextran-hydrolyzing enzymes. IPU is highly glycosylated, making it difficult to obtain its crystal. We used endoglycosidase H(f) to cleave the N-linked oligosaccharides of IPU, and we here determined the unliganded and isopanose-complexed forms of IPU, both solved at 1.7-A resolution. IPU is composed of domains N and C joined by a short linker, with electron density maps for 11 or 12 N-acetylglucosamine residues per molecule. Domain N consists of 13 beta-strands and forms a beta-sandwich. Domain C, where the active site is located, forms a right-handed beta-helix, and the lengths of the pitches of each coil of the beta-helix are similar to those of GH49 dextranase and GH28 polygalacturonase. The entire structure of IPU resembles that of a GH49 enzyme, Penicillium minioluteum dextranase (Dex49A), despite a difference in substrate specificity. Compared with the active sites of IPU and Dex49A, the amino acid residues participating in subsites +2 and +3 are not conserved, and the glucose residues of isopanose bound to IPU completely differ in orientation from the corresponding glucose residues of isomaltose bound to Dex49A. The shape of the catalytic cleft characterized by the seventh coil of the beta-helix and a loop from domain N appears to be critical in determining the specificity of IPU for pullulan.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z8/2z8g_consurf.spt"</scriptWhenChecked>
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2Z8G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8G OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49., Mizuno M, Koide A, Yamamura A, Akeboshi H, Yoshida H, Kamitori S, Sakano Y, Nishikawa A, Tonozuka T, J Mol Biol. 2008 Feb 8;376(1):210-20. Epub 2007 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18155243 18155243]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z8g ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Isopullulanase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Akeboshi H]]
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[[Category: Akeboshi, H.]]
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[[Category: Kamitori S]]
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[[Category: Kamitori, S.]]
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[[Category: Koide A]]
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[[Category: Koide, A.]]
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[[Category: Mizuno M]]
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[[Category: Mizuno, M.]]
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[[Category: Nishikawa A]]
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[[Category: Nishikawa, A.]]
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[[Category: Sakano Y]]
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[[Category: Sakano, Y.]]
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[[Category: Tonozuka T]]
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[[Category: Tonozuka, T.]]
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[[Category: Yamamura A]]
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[[Category: Yamamura, A.]]
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[[Category: Yoshida H]]
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[[Category: Yoshida, H.]]
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[[Category: NAG]]
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[[Category: beta-helix]]
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[[Category: dextranase]]
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[[Category: gh49]]
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[[Category: glycoprotein]]
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[[Category: glycosidase]]
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[[Category: hydrolase]]
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[[Category: isopullulanase]]
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[[Category: pullulan]]
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[[Category: secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:52:50 2008''
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Current revision

Aspergillus niger ATCC9642 isopullulanase complexed with isopanose

PDB ID 2z8g

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