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35c8

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[[Image:35c8.gif|left|200px]]
 
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{{Structure
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==CATALYTIC ANTIBODY 5C8, FAB-INHIBITOR COMPLEX==
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|PDB= 35c8 |SIZE=350|CAPTION= <scene name='initialview01'>35c8</scene>, resolution 2.00&Aring;
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<StructureSection load='35c8' size='340' side='right'caption='[[35c8]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NOX:N-(PARA-GLUTARAMIDOPHENYL-ETHYL)-PIPERIDINIUM-N-OXIDE'>NOX</scene>
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<table><tr><td colspan='2'>[[35c8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=35C8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=35C8 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NOX:N-(PARA-GLUTARAMIDOPHENYL-ETHYL)-PIPERIDINIUM-N-OXIDE'>NOX</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=35c8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=35c8 OCA], [https://pdbe.org/35c8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=35c8 RCSB], [https://www.ebi.ac.uk/pdbsum/35c8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=35c8 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=35c8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=35c8 OCA], [http://www.ebi.ac.uk/pdbsum/35c8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=35c8 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/IGKC_MOUSE IGKC_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/5c/35c8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=35c8 ConSurf].
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<div style="clear:both"></div>
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'''CATALYTIC ANTIBODY 5C8, FAB-INHIBITOR COMPLEX'''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The catalysis of disfavored chemical reactions, especially those with no known natural enzyme counterparts, is one of the most promising achievements of catalytic antibody research. Antibodies 5C8, 14B9, 17F6, and 26D9, elicited by two different transition-state analogues, catalyze disfavored endo-tet cyclization reactions of trans-epoxy alcohols, in formal violation of Baldwin's rules for ring closure. Thus far, neither chemical nor enzyme catalysis has been capable of emulating the extraordinary activity and specificity of these antibodies. X-ray structures of two complexes of Fab 5C8 with the original hapten and with an inhibitor have been determined to 2.0 A resolution. The Fab structure has an active site that contains a putative catalytic diad, consisting of AspH95 and HisL89, capable of general acid/base catalysis. The stabilization of a positive charge that develops along the reaction coordinate appears to be an important factor for rate enhancement and for directing the reaction along the otherwise disfavored pathway. Sequence analysis of the four catalytic antibodies, as well as four inactive antibodies that strongly bind the transition-state analogues, suggests a conserved catalytic mechanism. The occurrence of the putative base HisL89 in all active antibodies, its absence in three out of the four analyzed inactive antibodies, and the rarity of a histidine at this position in immunoglobulins support an important catalytic role for this residue.
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[[Category: Large Structures]]
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==About this Structure==
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35C8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=35C8 OCA].
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==Reference==
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Structural basis for antibody catalysis of a disfavored ring closure reaction., Gruber K, Zhou B, Houk KN, Lerner RA, Shevlin CG, Wilson IA, Biochemistry. 1999 Jun 1;38(22):7062-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10353817 10353817]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Gruber K]]
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[[Category: Gruber, K.]]
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[[Category: Wilson IA]]
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[[Category: Wilson, I A.]]
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[[Category: catalytic antibody]]
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[[Category: fab]]
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[[Category: ring closure reaction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:44 2008''
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Current revision

CATALYTIC ANTIBODY 5C8, FAB-INHIBITOR COMPLEX

PDB ID 35c8

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