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4khp

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==Structure of the Thermus thermophilus 30S ribosomal subunit in complex with de-6-MSA-pactamycin==
==Structure of the Thermus thermophilus 30S ribosomal subunit in complex with de-6-MSA-pactamycin==
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<StructureSection load='4khp' size='340' side='right' caption='[[4khp]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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<StructureSection load='4khp' size='340' side='right'caption='[[4khp]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4khp]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KHP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KHP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4khp]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KHP FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=PCY:PACTAMYCIN'>PCY</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hnx|1hnx]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=PCY:PACTAMYCIN'>PCY</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4khp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4khp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4khp RCSB], [http://www.ebi.ac.uk/pdbsum/4khp PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4khp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4khp OCA], [https://pdbe.org/4khp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4khp RCSB], [https://www.ebi.ac.uk/pdbsum/4khp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4khp ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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Biosynthetically and chemically derived analogs of the antibiotic pactamycin and de-6-methylsalicylyl (MSA)-pactamycin have attracted recent interest as potential antiprotozoal and antitumor drugs. Here, we report a 3.1-A crystal structure of de-6-MSA-pactamycin bound to its target site on the Thermus thermophilus 30S ribosomal subunit. Although de-6-MSA-pactamycin lacks the MSA moiety, it shares the same binding site as pactamycin and induces a displacement of nucleic acid template bound at the E-site of the 30S. The structure highlights unique interactions between this pactamycin analog and the ribosome, which paves the way for therapeutic development of related compounds.
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Crystal Structure of a Bioactive Pactamycin Analog Bound to the 30S Ribosomal Subunit.,Tourigny DS, Fernandez IS, Kelley AC, Vakiti RR, Chattopadhyay AK, Dorich S, Hanessian S, Ramakrishnan V J Mol Biol. 2013 May 20. pii: S0022-2836(13)00303-3. doi:, 10.1016/j.jmb.2013.05.004. PMID:23702293<ref>PMID:23702293</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Thermus thermophilus]]
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[[Category: Large Structures]]
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[[Category: Fernandez, I S]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Kelley, A C]]
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[[Category: Fernandez IS]]
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[[Category: Ramakrishnan, V]]
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[[Category: Kelley AC]]
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[[Category: Tourigny, D S]]
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[[Category: Ramakrishnan V]]
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[[Category: Antibiotic]]
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[[Category: Tourigny DS]]
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[[Category: De-6-msa-pactamycin and pactamycin analog]]
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[[Category: E site]]
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[[Category: Mrna]]
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[[Category: Pactamycin]]
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[[Category: Ribosome]]
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[[Category: Ribosome-antibiotic complex]]
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[[Category: Translation]]
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[[Category: Trna]]
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Current revision

Structure of the Thermus thermophilus 30S ribosomal subunit in complex with de-6-MSA-pactamycin

PDB ID 4khp

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