4qf1

<StructureSection load='4qf1' size='340' side='right' caption='[[4qf1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>

<table><tr><td colspan='2'>[[4qf1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QF1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QF1 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hpy|4hpy]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qf1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qf1 RCSB], [http://www.ebi.ac.uk/pdbsum/4qf1 PDBsum]</span></td></tr>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

In HIV-1, the ability to mount antibody responses to conserved, neutralizing epitopes is critical for protection. Here we have studied the light chain usage of human and rhesus macaque antibodies targeted to a dominant region of the HIV-1 envelope second variable (V2) region involving lysine (K) 169, the site of immune pressure in the RV144 vaccine efficacy trial. We found that humans and rhesus macaques used orthologous lambda variable gene segments encoding a glutamic acid-aspartic acid (ED) motif for K169 recognition. Structure determination of an unmutated ancestor antibody demonstrated that the V2 binding site was preconfigured for ED motif-mediated recognition prior to maturation. Thus, light chain usage for recognition of the site of immune pressure in the RV144 trial is highly conserved across species. These data indicate that the HIV-1 K169-recognizing ED motif has persisted over the diversification between rhesus macaques and humans, suggesting an evolutionary advantage of this antibody recognition mode.

Antibody light-chain-restricted recognition of the site of immune pressure in the RV144 HIV-1 vaccine trial is phylogenetically conserved.,Wiehe K, Easterhoff D, Luo K, Nicely NI, Bradley T, Jaeger FH, Dennison SM, Zhang R, Lloyd KE, Stolarchuk C, Parks R, Sutherland LL, Scearce RM, Morris L, Kaewkungwal J, Nitayaphan S, Pitisuttithum P, Rerks-Ngarm S, Sinangil F, Phogat S, Michael NL, Kim JH, Kelsoe G, Montefiori DC, Tomaras GD, Bonsignori M, Santra S, Kepler TB, Alam SM, Moody MA, Liao HX, Haynes BF Immunity. 2014 Dec 18;41(6):909-18. doi: 10.1016/j.immuni.2014.11.014. Epub 2014 , Nov 29. PMID:25526306<ref>PMID:25526306</ref>

== References ==

[[Category: Alam, S M]]

[[Category: Bonsignori, M]]

[[Category: Bradley, T]]

[[Category: Dennison, S M]]

[[Category: Easterhoff, D]]

[[Category: Haynes, B F]]

[[Category: Jaeger, F H]]

[[Category: Kaewkungwal, J]]

[[Category: Kelsoe, G]]

[[Category: Kepler, T B]]

[[Category: Kim, J]]

[[Category: Liao, H X]]

[[Category: Lloyd, K E]]

[[Category: Luo, K]]

[[Category: Michael, N]]

[[Category: Montefiori, D C]]

[[Category: Moody, M A]]

[[Category: Morris, L]]

[[Category: Nicely, N I]]

[[Category: Nitayaphan, S]]

[[Category: Parks, R]]

[[Category: Pitisuttithum, P]]

[[Category: Rerks-Ngarm, S]]

[[Category: Santra, S]]

[[Category: Scearce, R M]]

[[Category: Stolarchuk, C]]

[[Category: Sutherland, L L]]

[[Category: Tomaras, G]]

[[Category: Wiehe, K]]

[[Category: Zhang, R]]

[[Category: Immune system]]