5jva

<table><tr><td colspan='2'>[[5jva]] is a 4 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3t6h 3t6h]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JVA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JVA FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3t6h|3t6h]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jva OCA], [http://pdbe.org/5jva PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jva RCSB], [http://www.ebi.ac.uk/pdbsum/5jva PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jva ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

The red fluorescent protein variant TagRFP-T has greatly improved photostability over its parent molecule, TagRFP, but the underlying mechanism leading to this improvement is to date unknown. The 1.95 A resolution crystallographic structure of TagRFP-T showed that its chromophore exists as a mixture of cis and trans coplanar isomers in roughly equal proportions. Interestingly, both isomers are able to fluoresce, a property that has never been observed in any other fluorescent protein. We propose a "circular restoration model" for TagRFP-T to explain its superior photostability: There are four co-existing chromophore states (cis/trans protonated/ionized state) that can be driven by light to transform from one state into another. This model also explains how TagRPF-T essentially eliminates the temporary dark state (reversible photobleaching).

The crystal structure of red fluorescent protein TagRFP-T reveals the mechanism of its superior photostability.,Liu R, Liang QN, Du SQ, Hu XJ, Ding Y Biochem Biophys Res Commun. 2016 Aug 19;477(2):229-34. doi:, 10.1016/j.bbrc.2016.06.047. Epub 2016 Jun 11. PMID:27297107<ref>PMID:27297107</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 5jva" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Hu, X J]]

[[Category: Beta barrel]]

[[Category: Tagrfp-t]]