1rh4
From Proteopedia
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[[Image:1rh4.gif|left|200px]] | [[Image:1rh4.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1rh4| PDB=1rh4 | SCENE= }} | |
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'''RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER''' | '''RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | The following page contains interesting information on the relation of 1RH4 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH4 OCA]. | |
==Reference== | ==Reference== | ||
High-resolution protein design with backbone freedom., Harbury PB, Plecs JJ, Tidor B, Alber T, Kim PS, Science. 1998 Nov 20;282(5393):1462-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9822371 9822371] | High-resolution protein design with backbone freedom., Harbury PB, Plecs JJ, Tidor B, Alber T, Kim PS, Science. 1998 Nov 20;282(5393):1462-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9822371 9822371] | ||
[[Category: Designer Proteins]] | [[Category: Designer Proteins]] | ||
| - | [[Category: Protein complex]] | ||
| - | [[Category: Synthetic construct]] | ||
[[Category: Alber, T.]] | [[Category: Alber, T.]] | ||
[[Category: Harbury, P B.]] | [[Category: Harbury, P B.]] | ||
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[[Category: Plecs, J J.]] | [[Category: Plecs, J J.]] | ||
[[Category: Tidor, B.]] | [[Category: Tidor, B.]] | ||
| - | [[Category: | + | [[Category: Coiled coil]] |
| - | [[Category: | + | [[Category: De novo design]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:29:31 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:29, 3 May 2008
RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER
Overview
Recent advances in computational techniques have allowed the design of precise side-chain packing in proteins with predetermined, naturally occurring backbone structures. Because these methods do not model protein main-chain flexibility, they lack the breadth to explore novel backbone conformations. Here the de novo design of a family of alpha-helical bundle proteins with a right-handed superhelical twist is described. In the design, the overall protein fold was specified by hydrophobic-polar residue patterning, whereas the bundle oligomerization state, detailed main-chain conformation, and interior side-chain rotamers were engineered by computational enumerations of packing in alternate backbone structures. Main-chain flexibility was incorporated through an algebraic parameterization of the backbone. The designed peptides form alpha-helical dimers, trimers, and tetramers in accord with the design goals. The crystal structure of the tetramer matches the designed structure in atomic detail.
About this Structure
The following page contains interesting information on the relation of 1RH4 with [Designer Proteins]. Full crystallographic information is available from OCA.
Reference
High-resolution protein design with backbone freedom., Harbury PB, Plecs JJ, Tidor B, Alber T, Kim PS, Science. 1998 Nov 20;282(5393):1462-7. PMID:9822371 Page seeded by OCA on Sat May 3 07:29:31 2008
