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1i21

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(New page: 200px<br /><applet load="1i21" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i21, resolution 2.40&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1i21.jpg|left|200px]]<br /><applet load="1i21" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i21, resolution 2.40&Aring;" />
 
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'''CRYSTAL STRUCTURE OF YEAST GNA1'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF YEAST GNA1==
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The yeast enzymes involved in UDP-GlcNAc biosynthesis are potential, targets for antifungal agents. GNA1, a novel member of the Gcn5-related, N-acetyltransferase (GNAT) superfamily, participates in UDP-GlcNAc, biosynthesis by catalyzing the formation of GlcNAc6P from AcCoA and, GlcN6P. We have solved three crystal structures corresponding to the apo, Saccharomyces cerevisiae GNA1, the GNA1-AcCoA, and the GNA1-CoA-GlcNAc6P, complexes and have refined them to 2.4, 1.3, and 1.8 A resolution, respectively. These structures not only reveal a stable, beta-intertwined, dimeric assembly with the GlcNAc6P binding site located at the dimer, interface but also shed light on the catalytic machinery of GNA1 at an, atomic level. Hence, they broaden our understanding of structural features, required for GNAT activity, provide structural details for related, aminoglycoside N-acetyltransferases, and highlight the adaptability of the, GNAT superfamily members to acquire various specificities.
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<StructureSection load='1i21' size='340' side='right'caption='[[1i21]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1i21]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I21 FirstGlance]. <br>
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1I21 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Glucosamine_6-phosphate_N-acetyltransferase Glucosamine 6-phosphate N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.4 2.3.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I21 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i21 OCA], [https://pdbe.org/1i21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i21 RCSB], [https://www.ebi.ac.uk/pdbsum/1i21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i21 ProSAT]</span></td></tr>
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The crystal structures of Apo and complexed Saccharomyces cerevisiae GNA1 shed light on the catalytic mechanism of an amino-sugar N-acetyltransferase., Peneff C, Mengin-Lecreulx D, Bourne Y, J Biol Chem. 2001 May 11;276(19):16328-34. Epub 2001 Feb 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11278591 11278591]
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</table>
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[[Category: Glucosamine 6-phosphate N-acetyltransferase]]
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== Function ==
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[https://www.uniprot.org/uniprot/GNA1_YEAST GNA1_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i2/1i21_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i21 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Bourne Y]]
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[[Category: Bourne, Y.]]
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[[Category: Mengin-Lecreulx D]]
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[[Category: Mengin-Lecreulx, D.]]
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[[Category: Peneff C]]
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[[Category: Peneff, C.]]
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[[Category: alpha/beta]]
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[[Category: domain swapping]]
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[[Category: gnat]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:59:09 2007''
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Current revision

CRYSTAL STRUCTURE OF YEAST GNA1

PDB ID 1i21

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