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1ieh

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(New page: 200px<br /> <applet load="1ieh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ieh" /> '''SOLUTION STRUCTURE OF A SOLUBLE SINGLE-DOMA...)
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[[Image:1ieh.gif|left|200px]]<br />
 
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<applet load="1ieh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ieh" />
 
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'''SOLUTION STRUCTURE OF A SOLUBLE SINGLE-DOMAIN ANTIBODY WITH HYDROPHOBIC RESIDUES TYPICAL OF A VL/VH INTERFACE'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF A SOLUBLE SINGLE-DOMAIN ANTIBODY WITH HYDROPHOBIC RESIDUES TYPICAL OF A VL/VH INTERFACE==
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The three-dimensional structure of a llama single-domain antibody BrucD4-4, was established by use of solution NMR spectroscopy. BrucD4-4 has Val, Gly, Leu, and Trp residues at positions 37, 44, 45, and 47, which are, considered to be a hallmark to distinguish llama VH from V(H)H fragments, at the germline level. In contrast to the murine and human VHs, BrucD4-4, has sufficient solubility, is monomeric in solution, and displays, high-quality NMR spectra characteristic of well-structured proteins. Amide, proton/deuterium exchange and the (15)N relaxation data showed that, BrucD4-4 has a classic protein structure with a well-packed core and, comparatively mobile surface loops. The three-dimensional architecture of, BrucD4-4 is analogous to that of VHs from murine and human F(v)s and, camelid V(H)Hs with two pleated beta-sheets formed by four and five, beta-strands. A canonical and undistorted beta-barrel exposes a number of, hydrophobic residues into the solvent on the surface of the, three-dimensional structure. The eight-residue H3 loop folds over the side, chain of Val37 similarly to that in llama V(H)Hs; however, this, interaction may be transient due to the H3 conformational flexibility., Overall, the surface characteristics of BrucD4-4 with respect to, hydrophobicity appear to lie between the human VH domain from Fv Pot and, the llama V(H)H fragment HC-V, which may explain its enhanced solubility, allowing NMR structural analysis.
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<StructureSection load='1ieh' size='340' side='right'caption='[[1ieh]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ieh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IEH FirstGlance]. <br>
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1IEH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IEH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ieh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ieh OCA], [https://pdbe.org/1ieh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ieh RCSB], [https://www.ebi.ac.uk/pdbsum/1ieh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ieh ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of a llama single-domain antibody with hydrophobic residues typical of the VH/VL interface., Vranken W, Tolkatchev D, Xu P, Tanha J, Chen Z, Narang S, Ni F, Biochemistry. 2002 Jul 9;41(27):8570-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12093273 12093273]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/1ieh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ieh ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Lama glama]]
[[Category: Lama glama]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Chen, Z.]]
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[[Category: Chen Z]]
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[[Category: Narang, S.]]
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[[Category: Narang S]]
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[[Category: Ni, F.]]
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[[Category: Ni F]]
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[[Category: Tanha, J.]]
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[[Category: Tanha J]]
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[[Category: Tolkatchev, D.]]
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[[Category: Tolkatchev D]]
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[[Category: Vranken, W.]]
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[[Category: Vranken W]]
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[[Category: Xu, P.]]
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[[Category: Xu P]]
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[[Category: immunoglobulin beta-barrel]]
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[[Category: two pleated beta-sheet]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:32:51 2007''
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Current revision

SOLUTION STRUCTURE OF A SOLUBLE SINGLE-DOMAIN ANTIBODY WITH HYDROPHOBIC RESIDUES TYPICAL OF A VL/VH INTERFACE

PDB ID 1ieh

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