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1ifq

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(New page: 200px<br /><applet load="1ifq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ifq, resolution 2.40&Aring;" /> '''Sec22b N-terminal do...)
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[[Image:1ifq.jpg|left|200px]]<br /><applet load="1ifq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ifq, resolution 2.40&Aring;" />
 
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'''Sec22b N-terminal domain'''<br />
 
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==Overview==
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==Sec22b N-terminal domain==
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Intra-cellular membrane fusion is facilitated by the association of SNAREs, from opposite membranes into stable alpha-helical bundles. Many SNAREs, in, addition to their alpha-helical regions, contain N-terminal domains that, likely have essential regulatory functions. To better understand this, regulation, we have determined the 2.4-A crystal structure of the, 130-amino acid N-terminal domain of mouse Sec22b (mSec22b), a SNARE, involved in endoplasmic reticulum/Golgi membrane trafficking. The domain, consists of a mixed alpha-helical/beta-sheet fold that resembles a, circular permutation of the actin/poly-proline binding protein, profilin, and the GAF/PAS family of regulatory modules. The structure is distinct, from the previously characterized N-terminal domain of syntaxin 1A, and, unlike syntaxin 1A, the N-terminal domain of mSec22b has no effect on the, rate of SNARE assembly in vitro. An analysis of surface conserved residues, reveals a potential protein interaction site. Key residues in this site, are distinct in two mammalian Sec22 variants that lack SNARE domains., Finally, sequence analysis indicates that a similar domain is likely, present in the endosomal/lysosomal SNARE VAMP7.
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<StructureSection load='1ifq' size='340' side='right'caption='[[1ifq]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ifq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IFQ FirstGlance]. <br>
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1IFQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IFQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ifq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ifq OCA], [https://pdbe.org/1ifq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ifq RCSB], [https://www.ebi.ac.uk/pdbsum/1ifq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ifq ProSAT]</span></td></tr>
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A novel snare N-terminal domain revealed by the crystal structure of Sec22b., Gonzalez LC Jr, Weis WI, Scheller RH, J Biol Chem. 2001 Jun 29;276(26):24203-11. Epub 2001 Apr 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11309394 11309394]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SC22B_MOUSE SC22B_MOUSE] SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/if/1ifq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ifq ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Gonzalez Jr LC]]
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[[Category: Jr., L.C.Gonzalez.]]
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[[Category: Scheller RH]]
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[[Category: Scheller, R.H.]]
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[[Category: Weis WI]]
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[[Category: Weis, W.I.]]
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[[Category: GOL]]
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[[Category: alpha/beta 3-layer sandwich]]
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[[Category: five-stranded anti-parallel beta sheet]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:22:18 2007''
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Current revision

Sec22b N-terminal domain

PDB ID 1ifq

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