We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1ikl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1ikl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ikl" /> '''NMR STUDY OF MONOMERIC HUMAN INTERLEUKIN-8 ...)
Current revision (07:47, 3 April 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ikl.gif|left|200px]]<br />
 
-
<applet load="1ikl" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ikl" />
 
-
'''NMR STUDY OF MONOMERIC HUMAN INTERLEUKIN-8 (MINIMIZED AVERAGE STRUCTURE)'''<br />
 
-
==Overview==
+
==NMR study of monomeric human interleukin-8 (minimized average structure)==
-
The solution structure of a monomeric form of interleukin-8 (IL-8) has, been solved using 1H NMR spectroscopy. The chemically synthesized, nonnatural analog [IL-8 (4-72) L25 NH--&gt;NCH3] has the same activity as, that of native IL-8. Thirty structures were generated using the hybrid, distance geometry and simulated annealing protocol using the program, X-PLOR. The structure is well-defined except for N-terminal residues 4-6, and C-terminal residues 67-72. The rms distribution about the average, structure for residues 7-66 is 0.38 A for the backbone atoms and 0.87 A, for all heavy atoms. The structure consists of a series of turns and loops, followed by a triple-stranded beta sheet and a C-terminal alpha helix. The, structure of the monomer is largely similar to the native dimeric IL-8, structures previously determined by both NMR and X-ray methods. The major, difference is that, in the monomeric analog, the C-terminal residues 67-72, are disordered whereas they are helical in the two dimeric structures. The, best fit superposition of the backbone atoms of residues 7-66 of the, monomer structure on the dimeric IL-8 structures showed rms differences of, 1.5 and 1.2 A respectively. The turn (residues 31-35), which is disulfide, linked to the N-terminal region, adopts a conformation in the monomer, similar to that seen in the dimeric X-ray structure (rms difference 1.4 A), and different from that seen in the dimeric NMR structure (rms difference, 2.7 A).(ABSTRACT TRUNCATED AT 250 WORDS)
+
<StructureSection load='1ikl' size='340' side='right'caption='[[1ikl]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ikl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IKL FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLE:N-METHYLLEUCINE'>MLE</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ikl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ikl OCA], [https://pdbe.org/1ikl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ikl RCSB], [https://www.ebi.ac.uk/pdbsum/1ikl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ikl ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/IL8_HUMAN IL8_HUMAN] IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.<ref>PMID:2145175</ref> <ref>PMID:2212672</ref> <ref>PMID:11978786</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ik/1ikl_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ikl ConSurf].
 +
<div style="clear:both"></div>
-
==Disease==
+
==See Also==
-
Known disease associated with this structure: AIDS, slow progression to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=146929 146929]]
+
*[[Interleukin 3D structures|Interleukin 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1IKL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CH3 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IKL OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
-
1H NMR solution structure of an active monomeric interleukin-8., Rajarathnam K, Clark-Lewis I, Sykes BD, Biochemistry. 1995 Oct 10;34(40):12983-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7548056 7548056]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Clark-Lewis, I.]]
+
[[Category: Clark-Lewis I]]
-
[[Category: Rajarathnam, K.]]
+
[[Category: Rajarathnam K]]
-
[[Category: Sykes, B.D.]]
+
[[Category: Sykes BD]]
-
[[Category: CH3]]
+
-
[[Category: cytokine (chemotactic)]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:30:50 2007''
+

Current revision

NMR study of monomeric human interleukin-8 (minimized average structure)

PDB ID 1ikl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ikl"
Personal tools